Combining multi-antigenic immunodot with indirect immunofluorescence on HEp-2 cells improves the diagnosis of systemic sclerosis.
Adult
Aged
Autoantibodies
/ blood
Autoantigens
/ immunology
Cell Line
Centromere Protein A
/ immunology
Centromere Protein B
/ immunology
DNA Topoisomerases, Type I
/ immunology
Female
Fluorescent Antibody Technique, Indirect
/ methods
Humans
Immunoblotting
/ methods
Male
Middle Aged
Retrospective Studies
Scleroderma, Systemic
/ diagnosis
HEp-2 cells
Immunodot
Luminex®
Systemic sclerosis
Journal
Clinical immunology (Orlando, Fla.)
ISSN: 1521-7035
Titre abrégé: Clin Immunol
Pays: United States
ID NLM: 100883537
Informations de publication
Date de publication:
08 2021
08 2021
Historique:
received:
11
02
2021
revised:
03
06
2021
accepted:
04
06
2021
pubmed:
11
6
2021
medline:
18
9
2021
entrez:
10
6
2021
Statut:
ppublish
Résumé
Systemic sclerosis (SSc) is associated, in nearly all patients, with autoantibodies (Ab). Accordingly, and in order to identify major (anti-CEN A/B and anti-Topo I) but also minor Abs, the usefulness of combining indirect immunofluorescence (IIF) on HEp-2 cells with an 11 multi-antigenic SSc immunodot was explored. 1689 samples tested at the request of clinicians, were evaluated retrospectively. The positivity rate was 28.8% and the diagnosis of SSc was supported for 232 samples. Two groups of Abs were considered: group 1, Abs (anti-CENP A/B, anti-Topo I) present at elevated levels in SSc patients; group 2, Abs for which the Ab specificity (odds ratio and/or positive predictive value) was improved by using IIF on HEp-2 cells (RNA-Polymerase III, fibrillarin, Th/T0, PM-Scl). Altogether, this study highlights the utility of combining IIF on HEp-2 cells with the SSc immunodot as the first line of an SSc Abs detection/SSc diagnostic strategy.
Identifiants
pubmed: 34111525
pii: S1521-6616(21)00111-X
doi: 10.1016/j.clim.2021.108774
pii:
doi:
Substances chimiques
Autoantibodies
0
Autoantigens
0
CENPA protein, human
0
CENPB protein, human
0
Centromere Protein A
0
Centromere Protein B
0
DNA Topoisomerases, Type I
EC 5.99.1.2
TOP1 protein, human
EC 5.99.1.2
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108774Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.