AGO2 localizes to cytokinetic protrusions in a p38-dependent manner and is needed for accurate cell division.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
11 06 2021
11 06 2021
Historique:
received:
03
12
2019
accepted:
22
02
2021
entrez:
12
6
2021
pubmed:
13
6
2021
medline:
11
8
2021
Statut:
epublish
Résumé
Argonaute 2 (AGO2) is an indispensable component of the RNA-induced silencing complex, operating at the translational or posttranscriptional level. It is compartmentalized into structures such as GW- and P-bodies, stress granules and adherens junctions as well as the midbody. Here we show using immunofluorescence, image and bioinformatic analysis and cytogenetics that AGO2 also resides in membrane protrusions such as open- and close-ended tubes. The latter are cytokinetic bridges where AGO2 colocalizes at the midbody arms with cytoskeletal components such as α-Τubulin and Aurora B, and various kinases. AGO2, phosphorylated on serine 387, is located together with Dicer at the midbody ring in a manner dependent on p38 MAPK activity. We further show that AGO2 is stress sensitive and important to ensure the proper chromosome segregation and cytokinetic fidelity. We suggest that AGO2 is part of a regulatory mechanism triggered by cytokinetic stress to generate the appropriate micro-environment for local transcript homeostasis.
Identifiants
pubmed: 34117353
doi: 10.1038/s42003-021-02130-0
pii: 10.1038/s42003-021-02130-0
pmc: PMC8196063
doi:
Substances chimiques
AGO2 protein, human
0
Actins
0
Argonaute Proteins
0
Tubulin
0
p38 Mitogen-Activated Protein Kinases
EC 2.7.11.24
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
726Références
PLoS One. 2014 Jun 19;9(6):e99196
pubmed: 24945745
Metabolism. 2013 Oct;62(10):1387-93
pubmed: 23773982
Transl Res. 2014 Nov;164(5):359-65
pubmed: 24929208
Science. 2004 Aug 27;305(5688):1289-92
pubmed: 15297624
Genetics. 2016 Oct;204(2):543-553
pubmed: 27466226
J Biol Chem. 2010 Dec 17;285(51):40163-70
pubmed: 20956539
Nucleic Acids Res. 2015 Jul 1;43(W1):W460-6
pubmed: 25977294
Cell Rep. 2014 Jan 16;6(1):211-21
pubmed: 24388755
Int J Mol Sci. 2020 Apr 07;21(7):
pubmed: 32272708
PLoS One. 2016 Mar 01;11(3):e0150291
pubmed: 26930655
Mol Cell. 2004 Jul 23;15(2):185-97
pubmed: 15260970
Cell Cycle. 2012 Nov 15;11(22):4211-21
pubmed: 23095638
Int J Mol Sci. 2020 Sep 30;21(19):
pubmed: 33007856
J Microsc. 2013 Jan;249(1):13-25
pubmed: 23126323
Science. 2004 Feb 13;303(5660):1007-10
pubmed: 14963329
PLoS One. 2016 Aug 12;11(8):e0161165
pubmed: 27518285
Nature. 2007 Jul 5;448(7149):83-6
pubmed: 17589500
Microsc Res Tech. 2006 Apr;69(4):260-6
pubmed: 16586486
BMC Cancer. 2019 Jul 19;19(1):712
pubmed: 31324173
Nature. 2017 Feb 9;542(7640):197-202
pubmed: 28114302
Genetics. 2008 Mar;178(3):1311-25
pubmed: 18245345
J Histochem Cytochem. 1977 Jul;25(7):741-53
pubmed: 70454
Nature. 1991 Jan 17;349(6306):251-4
pubmed: 1702878
J Pathol. 2008 Jan;214(2):199-210
pubmed: 18161745
Biophys J. 2011 Apr 20;100(8):L37-9
pubmed: 21504718
BMC Syst Biol. 2013 Jul 23;7:66
pubmed: 23880086
Cell Cycle. 2011 Apr 15;10(8):1295-302
pubmed: 21474997
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Proc Natl Acad Sci U S A. 2006 Nov 28;103(48):18125-30
pubmed: 17116888
Methods. 2017 Feb 15;115:28-41
pubmed: 28057586
Mol Cell. 2007 Jun 8;26(5):611-23
pubmed: 17560368
J Cell Biol. 2017 Oct 2;216(10):3073-3085
pubmed: 28877994
Nucleic Acids Res. 2013 Jan;41(Database issue):D239-45
pubmed: 23193281
Nucleic Acids Res. 2012 Oct;40(19):9596-603
pubmed: 22848104
Nat Rev Mol Cell Biol. 2012 Mar 22;13(4):251-62
pubmed: 22436748
PLoS Genet. 2015 Feb 19;11(2):e1005013
pubmed: 25695507
Nat Cell Biol. 2009 Mar;11(3):328-36
pubmed: 19198598
Adv Exp Med Biol. 2013;768:165-82
pubmed: 23224970
Chromosome Res. 2003;11(2):137-46
pubmed: 12733640
Sci Rep. 2016 Jun 03;6:27085
pubmed: 27255932
Nat Rev Genet. 2013 Jul;14(7):447-59
pubmed: 23732335
Nature. 2010 Jan 28;463(7280):485-92
pubmed: 20110992
J Cell Biol. 2007 Jun 18;177(6):1017-27
pubmed: 17562790
Curr Opin Cell Biol. 2009 Dec;21(6):796-805
pubmed: 19836940
Int J Mol Sci. 2020 Jun 03;21(11):
pubmed: 32503341
Cancers (Basel). 2020 Mar 05;12(3):
pubmed: 32150835
Biochem J. 2008 Aug 1;413(3):429-36
pubmed: 18476811
Exp Cell Res. 2012 Jul 15;318(12):1428-34
pubmed: 22487096
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Cell Discov. 2015 Oct 20;1:15029
pubmed: 27462427
Nat Struct Mol Biol. 2010 Jan;17(1):17-23
pubmed: 19966796
Epigenetics. 2014 Jan;9(1):45-52
pubmed: 24149777
Genome Biol. 2008;9(2):210
pubmed: 18304383
Nature. 2012 Aug 9;488(7410):231-5
pubmed: 22722852
Plant Cell. 2007 Aug;19(8):2583-94
pubmed: 17675402
EMBO Rep. 2014 May;15(5):548-56
pubmed: 24723684
J Biol Chem. 2018 Jun 15;293(24):9311-9325
pubmed: 29735530
Nat Cell Biol. 2008 Feb;10(2):211-9
pubmed: 18193035
Nat Struct Mol Biol. 2006 Sep;13(9):787-92
pubmed: 16936728
Nat Struct Mol Biol. 2020 Sep;27(9):790-801
pubmed: 32661421
RNA Biol. 2014;11(1):18-24
pubmed: 24384674
J Cell Biol. 1998 Sep 21;142(6):1533-45
pubmed: 9744882
BMC Biol. 2003 Dec 12;1:2
pubmed: 14670085
Nucleic Acids Res. 2011 Apr;39(7):2727-41
pubmed: 21148147
Nat Struct Mol Biol. 2005 Feb;12(2):133-7
pubmed: 15643423
J Cell Sci. 2012 Jun 15;125(Pt 12):2844-52
pubmed: 22454512
J Microsc. 2013 Dec;252(3):295-302
pubmed: 24117417
Genes Dev. 2008 Oct 15;22(20):2773-85
pubmed: 18923076