Quorum sensing governs collective dendritic cell activation in vivo.
Animals
CD8-Positive T-Lymphocytes
/ physiology
Cell Count
Dendritic Cells
/ drug effects
Immunity, Innate
/ immunology
Inflammation
/ pathology
Interferon Regulatory Factor-7
/ genetics
Interferon Type I
/ metabolism
Lymph Nodes
/ cytology
Mice, Inbred C57BL
Mice, Mutant Strains
Mice, Transgenic
Poly I-C
/ pharmacology
Quorum Sensing
/ physiology
cytokine signaling
dendritic cell activation
quorum sensing
type I IFN
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
02 08 2021
02 08 2021
Historique:
revised:
10
05
2021
received:
29
10
2020
accepted:
14
05
2021
pubmed:
15
6
2021
medline:
30
11
2021
entrez:
14
6
2021
Statut:
ppublish
Résumé
Dendritic cell (DC) activation by viral RNA sensors such as TLR3 and MDA-5 is critical for initiating antiviral immunity. Optimal DC activation is promoted by type I interferon (IFN) signaling which is believed to occur in either autocrine or paracrine fashion. Here, we show that neither autocrine nor paracrine type I IFN signaling can fully account for DC activation by poly(I:C) in vitro and in vivo. By controlling the density of type I IFN-producing cells in vivo, we establish that instead a quorum of type I IFN-producing cells is required for optimal DC activation and that this process proceeds at the level of an entire lymph node. This collective behavior, governed by type I IFN diffusion, is favored by the requirement for prolonged cytokine exposure to achieve DC activation. Furthermore, collective DC activation was found essential for the development of innate and adaptive immunity in lymph nodes. Our results establish how collective rather than cell-autonomous processes can govern the initiation of immune responses.
Identifiants
pubmed: 34124789
doi: 10.15252/embj.2020107176
pmc: PMC8327941
doi:
Substances chimiques
Interferon Regulatory Factor-7
0
Interferon Type I
0
Irf7 protein, mouse
0
Poly I-C
O84C90HH2L
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e107176Informations de copyright
© 2021 The Authors.
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