Draft de novo Genome Assembly of the Elusive Jaguarundi, Puma yagouaroundi.
Puma yagouaroundi
10X Genomics Chromium
Felidae
genome annotation
linked reads
whole genome assembly
Journal
The Journal of heredity
ISSN: 1465-7333
Titre abrégé: J Hered
Pays: United States
ID NLM: 0375373
Informations de publication
Date de publication:
01 11 2021
01 11 2021
Historique:
received:
12
04
2021
accepted:
17
06
2021
pubmed:
20
6
2021
medline:
15
12
2021
entrez:
19
6
2021
Statut:
ppublish
Résumé
The Puma lineage within the family Felidae consists of 3 species that last shared a common ancestor around 4.9 million years ago. Whole-genome sequences of 2 species from the lineage were previously reported: the cheetah (Acinonyx jubatus) and the mountain lion (Puma concolor). The present report describes a whole-genome assembly of the remaining species, the jaguarundi (Puma yagouaroundi). We sequenced the genome of a male jaguarundi with 10X Genomics linked reads and assembled the whole-genome sequence. The assembled genome contains a series of scaffolds that reach the length of chromosome arms and is similar in scaffold contiguity to the genome assemblies of cheetah and puma, with a contig N50 = 100.2 kbp and a scaffold N50 = 49.27 Mbp. We assessed the assembled sequence of the jaguarundi genome using BUSCO, aligned reads of the sequenced individual and another published female jaguarundi to the assembled genome, annotated protein-coding genes, repeats, genomic variants and their effects with respect to the protein-coding genes, and analyzed differences of the 2 jaguarundis from the reference mitochondrial genome. The jaguarundi genome assembly and its annotation were compared in quality, variants, and features to the previously reported genome assemblies of puma and cheetah. Computational analyzes used in the study were implemented in transparent and reproducible way to allow their further reuse and modification.
Identifiants
pubmed: 34146095
pii: 6305920
doi: 10.1093/jhered/esab036
pmc: PMC8558579
doi:
Banques de données
Dryad
['10.5061/dryad.wstqjq2mf']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
540-548Informations de copyright
© The American Genetic Association. 2021.
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