Associations between a mixture of phenols and phthalates and child behaviour in a French mother-child cohort with repeated assessment of exposure.


Journal

Environment international
ISSN: 1873-6750
Titre abrégé: Environ Int
Pays: Netherlands
ID NLM: 7807270

Informations de publication

Date de publication:
11 2021
Historique:
received: 02 03 2021
revised: 10 05 2021
accepted: 04 06 2021
pubmed: 21 6 2021
medline: 3 9 2021
entrez: 20 6 2021
Statut: ppublish

Résumé

Synthetic phenols and phthalates can interfere with biological pathways involved in brain development. Despite the high within-subject temporal variability of urinary concentrations observed for their metabolites, studies investigating effects of phenols and phthalates on child behaviour often relied on a limited number of spot biospecimens to assess exposure. Besides, the majority did not consider mixture effects. To study the combined effect of prenatal exposure to synthetic phenols and phthalates on child behaviour using repeated exposure measurements. We assessed concentrations of 12 phenols, 13 phthalate and 2 non-phthalate plasticizer metabolites in within-subject pools of multiple urine samples (median = 21 samples per individual pool) collected at two distinct time points during pregnancy in 416 mother-child pairs from the French SEPAGES cohort. Child behaviour was evaluated at two years using the Child Behaviour Checklist 1.5-5 (CBCL). Associations between a mixture of biomarkers of exposure and externalizing and internalizing behaviour scores were studied using adjusted Weighted Quantile Sum (WQS) regressions with a repeated holdout validation (100 repetitions). The positive WQS indexes were associated with both the externalizing and internalizing behaviour scores in the whole population, indicating greater risk of behavioural problems. Stratification for child sex suggested stronger associations in girls than boys. On average, girls externalizing and internalizing scores increased by 3.67 points (95% CI: 1.24, 6.10) and 2.47 points (95 %CI: 0.60, 4.33) respectively, for an increase of one tertile in the WQS index, compared with 1.70 points (95 %CI: -0.42, 3.81) and 1.17 points (95 %CI: -0.50, 2.84) in boys. Main contributors for the associations observed in girls were bisphenol A (weight of 18%), triclosan (17%) and monoethyl phthalate (MEP, 15%) for the externalizing score and MEP (19%), mono-benzyl phthalate (MBzP, 19%) and mono-n-butyl phthalate (MnBP, 16%) for the internalizing score. Our results suggest adverse associations between in utero exposure to a mixture of phenols and phthalates and child behaviour, mainly in girls. Public health consequences may be substantial due to the widespread exposure of the population to these compounds.

Sections du résumé

BACKGROUND
Synthetic phenols and phthalates can interfere with biological pathways involved in brain development. Despite the high within-subject temporal variability of urinary concentrations observed for their metabolites, studies investigating effects of phenols and phthalates on child behaviour often relied on a limited number of spot biospecimens to assess exposure. Besides, the majority did not consider mixture effects.
OBJECTIVES
To study the combined effect of prenatal exposure to synthetic phenols and phthalates on child behaviour using repeated exposure measurements.
METHODS
We assessed concentrations of 12 phenols, 13 phthalate and 2 non-phthalate plasticizer metabolites in within-subject pools of multiple urine samples (median = 21 samples per individual pool) collected at two distinct time points during pregnancy in 416 mother-child pairs from the French SEPAGES cohort. Child behaviour was evaluated at two years using the Child Behaviour Checklist 1.5-5 (CBCL). Associations between a mixture of biomarkers of exposure and externalizing and internalizing behaviour scores were studied using adjusted Weighted Quantile Sum (WQS) regressions with a repeated holdout validation (100 repetitions).
RESULTS
The positive WQS indexes were associated with both the externalizing and internalizing behaviour scores in the whole population, indicating greater risk of behavioural problems. Stratification for child sex suggested stronger associations in girls than boys. On average, girls externalizing and internalizing scores increased by 3.67 points (95% CI: 1.24, 6.10) and 2.47 points (95 %CI: 0.60, 4.33) respectively, for an increase of one tertile in the WQS index, compared with 1.70 points (95 %CI: -0.42, 3.81) and 1.17 points (95 %CI: -0.50, 2.84) in boys. Main contributors for the associations observed in girls were bisphenol A (weight of 18%), triclosan (17%) and monoethyl phthalate (MEP, 15%) for the externalizing score and MEP (19%), mono-benzyl phthalate (MBzP, 19%) and mono-n-butyl phthalate (MnBP, 16%) for the internalizing score.
DISCUSSION
Our results suggest adverse associations between in utero exposure to a mixture of phenols and phthalates and child behaviour, mainly in girls. Public health consequences may be substantial due to the widespread exposure of the population to these compounds.

Identifiants

pubmed: 34147998
pii: S0160-4120(21)00322-6
doi: 10.1016/j.envint.2021.106697
pii:
doi:

Substances chimiques

Environmental Pollutants 0
Phenols 0
Phthalic Acids 0
phthalic acid 6O7F7IX66E

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

106697

Informations de copyright

Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.

Auteurs

Ariane Guilbert (A)

Team of Environmental Epidemiology Applied to Reproduction and Respiratory Health, Institute for Advanced Biosciences (IAB), Grenoble Alpes University, Inserm, CNRS, 38700 La Tronche, France. Electronic address: ariane.guilbert@univ-grenoble-alpes.fr.

Matthieu Rolland (M)

Team of Environmental Epidemiology Applied to Reproduction and Respiratory Health, Institute for Advanced Biosciences (IAB), Grenoble Alpes University, Inserm, CNRS, 38700 La Tronche, France. Electronic address: matthieu.rolland@univ-grenoble-alpes.fr.

Isabelle Pin (I)

Team of Environmental Epidemiology Applied to Reproduction and Respiratory Health, Institute for Advanced Biosciences (IAB), Grenoble Alpes University, Inserm, CNRS, 38700 La Tronche, France; Pediatric Department, Grenoble Alpes University Hospital, 38700 La Tronche, France. Electronic address: ipin@chu-grenoble.fr.

Cathrine Thomsen (C)

Norwegian Institute of Public Health, Oslo, Norway. Electronic address: Cathrine.Thomsen@fhi.no.

Amrit K Sakhi (AK)

Norwegian Institute of Public Health, Oslo, Norway. Electronic address: AmritKaur.Sakhi@fhi.no.

Azemira Sabaredzovic (A)

Norwegian Institute of Public Health, Oslo, Norway. Electronic address: Azemira.Sabaredzovic@fhi.no.

Rémy Slama (R)

Team of Environmental Epidemiology Applied to Reproduction and Respiratory Health, Institute for Advanced Biosciences (IAB), Grenoble Alpes University, Inserm, CNRS, 38700 La Tronche, France. Electronic address: remy.slama@univ-grenoble-alpes.fr.

Karine Guichardet (K)

Pediatric Department, Grenoble Alpes University Hospital, 38700 La Tronche, France. Electronic address: KGuichardet@chu-grenoble.fr.

Claire Philippat (C)

Team of Environmental Epidemiology Applied to Reproduction and Respiratory Health, Institute for Advanced Biosciences (IAB), Grenoble Alpes University, Inserm, CNRS, 38700 La Tronche, France. Electronic address: claire.philippat@univ-grenoble-alpes.fr.

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