Corticosteroid Biosynthesis Revisited: No Direct Hydroxylation of Pregnenolone by Steroid 21-Hydroxylase.
17-alpha-Hydroxypregnenolone
/ metabolism
Adrenal Cortex Hormones
/ metabolism
Catalytic Domain
Cytochrome P-450 Enzyme System
Gas Chromatography-Mass Spectrometry
Humans
Hydroxylation
Models, Molecular
Molecular Docking Simulation
Pregnenolone
/ metabolism
Progesterone
/ metabolism
Schizosaccharomyces
Steroid 17-alpha-Hydroxylase
/ metabolism
Steroid 21-Hydroxylase
/ metabolism
Steroids
/ metabolism
Substrate Specificity
CYP21A2
GC-MS
corticosteroid
cytochrome P450
fission yeast (Schizosaccharomyces pombe)
molecular docking
steroid biosynthesis
Journal
Frontiers in endocrinology
ISSN: 1664-2392
Titre abrégé: Front Endocrinol (Lausanne)
Pays: Switzerland
ID NLM: 101555782
Informations de publication
Date de publication:
2021
2021
Historique:
received:
26
11
2020
accepted:
11
05
2021
entrez:
21
6
2021
pubmed:
22
6
2021
medline:
28
12
2021
Statut:
epublish
Résumé
Cytochrome P450s (CYPs) are an essential family of enzymes in the human body. They play a crucial role in metabolism, especially in human steroid biosynthesis. Reactions catalyzed by these enzymes are highly stereo- and regio-specific. Lack or severe malfunctions of CYPs can cause severe diseases and even shorten life. Hence, investigations on metabolic reactions and structural requirements of substrates are crucial to gain further knowledge on the relevance of different enzymes in the human body functions and the origin of diseases. One key enzyme in the biosynthesis of gluco- and mineralocorticoids is CYP21A2, also known as steroid 21-hydroxylase. To investigate the steric and regional requirements of substrates for this enzyme, we performed whole-cell biotransformation assays using a strain of fission yeast
Identifiants
pubmed: 34149610
doi: 10.3389/fendo.2021.633785
pmc: PMC8211424
doi:
Substances chimiques
Adrenal Cortex Hormones
0
Steroids
0
17-alpha-Hydroxypregnenolone
387-79-1
Progesterone
4G7DS2Q64Y
Pregnenolone
73R90F7MQ8
Cytochrome P-450 Enzyme System
9035-51-2
CYP21A2 protein, human
EC 1.14.14.16
Steroid 21-Hydroxylase
EC 1.14.14.16
Steroid 17-alpha-Hydroxylase
EC 1.14.14.19
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
633785Informations de copyright
Copyright © 2021 Loke, Stoll, Machalz, Botrè, Wolber, Bureik and Parr.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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