Effect of metronidazole on vaginal microbiota associated with asymptomatic bacterial vaginosis.
16s rRNA gene sequencing
Lactobacillus
bacteria
community
microbiota dysbiosis
Journal
Access microbiology
ISSN: 2516-8290
Titre abrégé: Access Microbiol
Pays: England
ID NLM: 101746927
Informations de publication
Date de publication:
2021
2021
Historique:
received:
05
11
2020
accepted:
17
03
2021
entrez:
21
6
2021
pubmed:
22
6
2021
medline:
22
6
2021
Statut:
epublish
Résumé
Vaginal dysbiosis-induced by an overgrowth of anaerobic bacteria is referred to as bacterial vaginosis (BV). The dysbiosis is associated with an increased risk for acquisition of sexually transmitted infections. Women with symptomatic BV are treated with oral metronidazole (MET), but its effectiveness remains to be elucidated. This study used whole-genome sequencing (WGS) to determine the changes in the microbiota among women treated with MET. WGS was conducted on DNA obtained from 20 vaginal swabs collected at four time points over 12 months from five randomly selected African American (AA) women. The baseline visit included all women who were diagnosed with asymptomatic BV and were untreated. All subjects were tested subsequently once every 2 months and received a course of MET for each BV episode during the 12 months. The BV status was classified according to Nugent scores (NSs) of vaginal smears. The microbial and resistome profiles were analysed along with the sociodemographic metadata. Despite treatment, none of the five participants reverted to normal vaginal flora - two were consistently positive for BV, and the rest experienced episodic cases of BV. WGS analyses showed
Identifiants
pubmed: 34151180
doi: 10.1099/acmi.0.000226
pii: 000226
pmc: PMC8209634
doi:
Types de publication
Journal Article
Langues
eng
Pagination
000226Informations de copyright
© 2021 The Authors.
Déclaration de conflit d'intérêts
The authors declare that there are no conflicts of interest.
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