Characterizing the proteome of bullous pemphigoid blister fluid utilizing tandem mass tag labeling coupled with LC-MS/MS.
Autoimmune blistering disorder
Blister fluid
Pemphigoid
Proteomics
Quantitative proteomics
Journal
Archives of dermatological research
ISSN: 1432-069X
Titre abrégé: Arch Dermatol Res
Pays: Germany
ID NLM: 8000462
Informations de publication
Date de publication:
Nov 2022
Nov 2022
Historique:
received:
17
02
2021
accepted:
05
06
2021
revised:
03
05
2021
pubmed:
22
6
2021
medline:
28
9
2022
entrez:
21
6
2021
Statut:
ppublish
Résumé
Bullous pemphigoid is an autoimmune blistering disease caused by autoantibodies against components of the cutaneous basement membrane zone. Autoantibodies lead to complement-dependent and -independent inflammation and blistering. Blister fluid is a valuable biologic resource, as it provides insight into both systemic and local microenvironment responses. Here, we utilized liquid chromatography with tandem mass spectrometry to characterize the bullous pemphigoid blister fluid proteome. We then depleted exosomes to better understand the exosomal versus non-exosomal proteome. We identified 339 proteins in the blister fluid of bullous pemphigoid patients. Gene ontology demonstrated enrichment of several key biologic processes including innate immune response, neutrophil degranulation, platelet degranulation, and complement activation. Exosome depletion resulted in a significant decrease in normalized reporter intensities of 192 proteins, consistent with our observation of a large number of exosomal proteins found in the blister fluid. We then compared the bullous pemphigoid blister fluid proteome to prior proteomic datasets in suction blister fluid, snake bites, and thermal burns, identifying 76 proteins unique to bullous pemphigoid. These include major basic protein, eosinophil peroxidase, galectin-10, and the immunoglobulin epsilon heavy constant region, consistent with tissue eosinophilia. We lastly validated several previously reported blister fluid exosomal components. Blister fluid in bullous pemphigoid contains a mixture of numerous biologic processes. While many of these processes are shared with blistering from alternative causes, we have identified several notable features unique to bullous pemphigoid.
Identifiants
pubmed: 34152480
doi: 10.1007/s00403-021-02253-8
pii: 10.1007/s00403-021-02253-8
doi:
Substances chimiques
Autoantibodies
0
Biological Products
0
Galectins
0
Proteome
0
Peroxidases
EC 1.11.1.-
Eosinophil Major Basic Protein
EC 3.1.27.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
921-928Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : FOR 2497
Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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