Ana1 helps recruit Polo to centrioles to promote mitotic PCM assembly and centriole elongation.
Ana1
Centriole
Centrosome
Cep295
PCM
PLK1
Pericentriolar material
Polo
Journal
Journal of cell science
ISSN: 1477-9137
Titre abrégé: J Cell Sci
Pays: England
ID NLM: 0052457
Informations de publication
Date de publication:
15 07 2021
15 07 2021
Historique:
received:
02
06
2021
accepted:
08
06
2021
pubmed:
23
6
2021
medline:
13
8
2021
entrez:
22
6
2021
Statut:
ppublish
Résumé
Polo kinase (PLK1 in mammals) is a master cell cycle regulator that is recruited to various subcellular structures, often by its polo-box domain (PBD), which binds to phosphorylated S-pS/pT motifs. Polo/PLK1 kinases have multiple functions at centrioles and centrosomes, and we have previously shown that in Drosophila phosphorylated Sas-4 initiates Polo recruitment to newly formed centrioles, while phosphorylated Spd-2 recruits Polo to the pericentriolar material (PCM) that assembles around mother centrioles in mitosis. Here, we show that Ana1 (Cep295 in humans) also helps to recruit Polo to mother centrioles in Drosophila. If Ana1-dependent Polo recruitment is impaired, mother centrioles can still duplicate, disengage from their daughters and form functional cilia, but they can no longer efficiently assemble mitotic PCM or elongate during G2. We conclude that Ana1 helps recruit Polo to mother centrioles to specifically promote mitotic centrosome assembly and centriole elongation in G2, but not centriole duplication, centriole disengagement or cilia assembly. This article has an associated First Person interview with the first author of the paper.
Identifiants
pubmed: 34156068
pii: 269170
doi: 10.1242/jcs.258987
pmc: PMC8325959
pii:
doi:
Substances chimiques
Cell Cycle Proteins
0
Drosophila Proteins
0
SPD-2 protein, Drosophila
0
polo protein, Drosophila
EC 2.7.11.-
Protein Serine-Threonine Kinases
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 104575
Pays : United Kingdom
Informations de copyright
© 2021. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing or financial interests.
Références
Mol Biol Cell. 2010 Nov 1;21(21):3617-29
pubmed: 20844083
Mol Cell. 2014 Aug 21;55(4):578-91
pubmed: 25042804
J Cell Biol. 1996 Dec;135(6 Pt 2):1701-13
pubmed: 8991084
J Cell Biol. 2009 Nov 2;187(3):355-63
pubmed: 19948479
Biol Open. 2012 Oct 15;1(10):965-76
pubmed: 23213374
Curr Biol. 2010 Dec 21;20(24):2178-86
pubmed: 21145741
Neuron. 1999 Mar;22(3):451-61
pubmed: 10197526
Nat Commun. 2015 Aug 21;6:8077
pubmed: 26293378
Curr Biol. 2015 Aug 3;25(15):R650-1
pubmed: 26241137
Elife. 2014 Aug 22;3:e03399
pubmed: 25149451
J Cell Biol. 2010 Nov 15;191(4):721-9
pubmed: 21059850
Proc Natl Acad Sci U S A. 2010 Dec 7;107(49):21022-7
pubmed: 21097701
Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9301-6
pubmed: 9689075
J Biol Chem. 2002 Aug 30;277(35):32282-93
pubmed: 12034729
J Cell Biol. 1998 Dec 14;143(6):1575-89
pubmed: 9852152
Cell Cycle. 2014;13(13):2064-72
pubmed: 24802643
J Cell Sci. 2011 Aug 1;124(Pt 15):2622-30
pubmed: 21750193
Curr Biol. 2010 Jul 27;20(14):1277-82
pubmed: 20656208
Neuron. 1994 Jun;12(6):1195-206
pubmed: 8011334
Curr Biol. 2007 Oct 23;17(20):1735-45
pubmed: 17935995
J Cell Sci. 2016 Jul 1;129(13):2501-13
pubmed: 27185865
Elife. 2019 Sep 09;8:
pubmed: 31498081
Cell. 2003 Oct 3;115(1):83-95
pubmed: 14532005
J Cell Sci. 2012 Dec 1;125(Pt 23):5881-6
pubmed: 22976301
Dev Cell. 2009 Sep;17(3):344-54
pubmed: 19758559
Nat Cell Biol. 2012 Nov;14(11):1148-58
pubmed: 23086237
J Cell Sci. 2003 Apr 1;116(Pt 7):1377-87
pubmed: 12615979
Cell. 2017 Jun 1;169(6):1078-1089.e13
pubmed: 28575671
Curr Biol. 2008 Jan 22;18(2):136-41
pubmed: 18207742
Mol Cell Biol. 1988 Nov;8(11):4727-35
pubmed: 2463465
Cell. 2003 Feb 21;112(4):575-87
pubmed: 12600319
Mol Biol Cell. 2006 Jan;17(1):448-59
pubmed: 16267267
Cytoskeleton (Hoboken). 2018 Nov;75(11):481-494
pubmed: 30414309
Curr Biol. 2007 Oct 23;17(20):1759-64
pubmed: 17919907
J Cell Biol. 2021 Apr 5;220(4):
pubmed: 33533934
J Cell Biol. 2014 Sep 29;206(7):855-65
pubmed: 25246616
Cell Rep. 2014 Aug 21;8(4):957-65
pubmed: 25131205
Biol Open. 2016 Oct 15;5(10):1431-1440
pubmed: 27591191
Development. 1999 Jul;126(13):2829-39
pubmed: 10357928
Curr Biol. 2014 Jun 2;24(11):1276-82
pubmed: 24835456
EMBO J. 2011 Jun 1;30(11):2233-45
pubmed: 21522128
Curr Biol. 2007 Sep 4;17(17):1498-503
pubmed: 17716897
Open Biol. 2018 Aug;8(8):
pubmed: 30135239
J Cell Biol. 2020 Jun 1;219(6):
pubmed: 32271878
Nat Cell Biol. 2012 Nov;14(11):1159-68
pubmed: 23086239
J Cell Biol. 2010 Nov 15;191(4):731-9
pubmed: 21059844
J Cell Biol. 2011 May 16;193(4):727-39
pubmed: 21576395
Nat Cell Biol. 2007 Apr;9(4):436-44
pubmed: 17351640
Dev Cell. 2019 Aug 5;50(3):355-366.e6
pubmed: 31303441
J Cell Sci. 2016 Oct 15;129(20):3732-3743
pubmed: 27577095
Nat Commun. 2015 Dec 09;6:10076
pubmed: 26647647
Nat Rev Mol Cell Biol. 2015 Oct;16(10):611-24
pubmed: 26373263
Dev Cell. 2010 Dec 14;19(6):913-9
pubmed: 21145506
Open Biol. 2012 Aug;2(8):120104
pubmed: 22977736
Bioinformatics. 2009 May 1;25(9):1189-91
pubmed: 19151095
J Cell Sci. 2016 Jul 1;129(13):2514-25
pubmed: 27206860
Dev Cell. 2016 Jun 20;37(6):545-57
pubmed: 27326932
J Cell Sci. 2020 Jun 22;133(12):
pubmed: 32409564
Cell. 2006 Jun 30;125(7):1375-86
pubmed: 16814722
Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1984-9
pubmed: 11854496
Mol Cell. 2006 Nov 3;24(3):409-22
pubmed: 17081991
Genetics. 2009 May;182(1):133-44
pubmed: 19293139
Dev Cell. 2014 Mar 31;28(6):659-69
pubmed: 24656740
J Biol Chem. 2004 May 14;279(20):21367-73
pubmed: 15016807
J Cell Biol. 2007 Aug 27;178(5):725-32
pubmed: 17709428
Curr Biol. 2011 Jul 12;21(13):1145-51
pubmed: 21723128
Cell. 2008 Jun 13;133(6):1032-42
pubmed: 18555779
EMBO J. 2000 Jan 17;19(2):241-52
pubmed: 10637228
Nat Commun. 2016 Aug 26;7:12567
pubmed: 27562453
PLoS One. 2014 Jan 22;9(1):e87016
pubmed: 24466316
J Cell Biol. 2003 Sep 1;162(5):863-75
pubmed: 12939256
J Cell Biol. 2011 Dec 26;195(7):1093-101
pubmed: 22184200
Nat Cell Biol. 2016 Jan;18(1):87-99
pubmed: 26595382
Science. 2003 Feb 21;299(5610):1228-31
pubmed: 12595692
Nat Commun. 2017 Aug 15;8(1):247
pubmed: 28811500
PLoS One. 2009 Jun 19;4(6):e5976
pubmed: 19543530
Sci Rep. 2018 May 15;8(1):7583
pubmed: 29765093
Curr Biol. 2007 Nov 20;17(22):1960-6
pubmed: 17980596
Sci Rep. 2015 Nov 03;5:15915
pubmed: 26525406
Science. 2015 May 15;348(6236):808-12
pubmed: 25977552
Mol Cell Biol. 2000 Jan;20(1):286-98
pubmed: 10594031
EMBO J. 2011 Jun 03;30(13):2634-47
pubmed: 21642957
Elife. 2015 Nov 04;4:
pubmed: 26530814
Mol Cell Biol. 2015 Aug;35(15):2626-40
pubmed: 26012549
Curr Biol. 2011 Aug 9;21(15):1259-67
pubmed: 21802300
Genetics. 2007 Mar;175(3):1505-31
pubmed: 17194782
Nat Commun. 2016 Aug 25;7:12476
pubmed: 27558293
Nature. 2010 Oct 7;467(7316):714-8
pubmed: 20852615