A rapid and inexpensive protocol to screen for third generation cephalosporin-resistant and non-fermenting Gram-negative rods directly in positive blood cultures.
Anti-Bacterial Agents
/ pharmacology
Bacteremia
/ blood
Bacterial Proteins
/ metabolism
Blood Culture
Cephalosporin Resistance
Cephalosporins
/ pharmacology
Gram-Negative Bacteria
/ drug effects
Gram-Negative Bacterial Infections
/ blood
Humans
Oxidoreductases
/ metabolism
Prospective Studies
Sensitivity and Specificity
beta-Lactamases
/ metabolism
Beta-Lacta Test
Gel separator tubes
Gram-negative rods bacteremia
Oxidase
Positive Blood culture
Third-generation cephalosporin resistance
Journal
Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
received:
21
01
2021
revised:
03
05
2021
accepted:
07
05
2021
pubmed:
27
6
2021
medline:
3
11
2021
entrez:
26
6
2021
Statut:
ppublish
Résumé
Third generation cephalosporins are frequently used in the first-line treatment of Gram-negative rod (GNR) bacteremia but are unsuitable in the case of extended-spectrum-beta-lactamase-producing Enterobacterales (ESBL-E) or non-fermenting GNR infections. The aim of this study was to develop and evaluate a simple and rapid two-test protocol involving oxidase and β-Lacta tests performed directly on positive blood culture broth as a preliminary screen for non-fermenting or third generation cephalosporins-resistant GNR. The diagnostic performance of this approach was evaluated on 294 bottles for the oxidase test and 267 bottles for the β-Lacta Test. The sensitivity and specificity of the oxidase test were respectively 93.1% and 100%, and the sensitivity of the β-Lacta Test for ESBL-E was 100% and the specificity 99.5%. This simple protocol, which can be implemented in all laboratories and performed in only 20 min, may be a valuable tool to optimize first-line antibiotic therapy for bacteremia.
Identifiants
pubmed: 34174522
pii: S0732-8893(21)00121-8
doi: 10.1016/j.diagmicrobio.2021.115428
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
Cephalosporins
0
Oxidoreductases
EC 1.-
beta-Lactamases
EC 3.5.2.6
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
115428Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no competing interests.