Quorum sensing inhibitory effect of bergamot oil and aspidosperma extract against Chromobacterium violaceum and Pseudomonas aeruginosa.
Anti-Bacterial Agents
/ pharmacology
Aspidosperma
/ chemistry
Biofilms
/ drug effects
Chromobacterium
/ drug effects
Gene Expression Regulation, Bacterial
/ drug effects
Plant Extracts
/ pharmacology
Plant Oils
/ pharmacology
Pseudomonas aeruginosa
/ drug effects
Quorum Sensing
/ drug effects
Virulence Factors
/ genetics
Anti-biofilm
Aspidosperma
Bergamot
Chromobacterium violaceum
Pseudomonas aeruginosa
Quorum sensing
Journal
Archives of microbiology
ISSN: 1432-072X
Titre abrégé: Arch Microbiol
Pays: Germany
ID NLM: 0410427
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
received:
29
03
2021
accepted:
21
06
2021
revised:
21
05
2021
pubmed:
28
6
2021
medline:
2
9
2021
entrez:
27
6
2021
Statut:
ppublish
Résumé
Quorum sensing (QS) represents a major target for reducing bacterial pathogenicity and antibiotic resistance. This study identifies bergamot and aspidosperma as new potential sources of anti-QS agents. We investigated the anti-QS activity of plant materials on both Chromobacterium violaceum and Pseudomonas aeruginosa. Initially, we determined the minimum inhibitory concentrations (MICs) of plant materials using a broth microdilution method. Subsequently, we tested the effect of sub-MIC concentrations on QS-regulated traits and virulence factors production in test bacteria. Results revealed that bergamot and aspidosperma inhibited the ability of C. violaceum to produce violacein. Other QS-controlled phenotypes of C. violaceum, namely chitinolytic activity, motility, and biofilm formation, were also reduced by both plant materials. Moreover, QS-linked traits of P. aeruginosa were also reduced. Bergamot inhibited swarming but not swimming motility, while aspidosperma diminished both motility types in P. aeruginosa. Both plant materials also demonstrated antibiofilm activity and inhibited the production of protease and pyocyanin in P. aeruginosa. Furthermore, we tested the anti-QS effect of plant materials on the transcriptional level using RT-qPCR. Bergamot dramatically downregulated the C. violaceum autoinducer synthase gene cviI and the vioB gene involved in violacein biosynthesis, confirming the phenotypic observation on its anti-QS activity. Aspidosperma also reduced the expression of cviI and vioB but less drastically than bergamot. In P. aeruginosa, downregulation in the transcripts of the QS genes lasI, lasR, rhlI, and rhlR was also achieved by bergamot and aspidosperma. Therefore, data in the present study suggest the usefulness of bergamot and aspidosperma as sources of antivirulence agents.
Identifiants
pubmed: 34175964
doi: 10.1007/s00203-021-02455-8
pii: 10.1007/s00203-021-02455-8
doi:
Substances chimiques
Anti-Bacterial Agents
0
Plant Extracts
0
Plant Oils
0
Virulence Factors
0
bergamot oil
39W1PKE3JI
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4663-4675Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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