Love is in the hair: arginine methylation of human hair proteins as novel cardiovascular biomarkers.

Arginine methylation (ArgMe) Asymmetric dimethylarginine (ADMA) Biomarker Cardiovascular disease (CVD) Hair Post-translational modifications (PTM) Protein

Journal

Amino acids
ISSN: 1438-2199
Titre abrégé: Amino Acids
Pays: Austria
ID NLM: 9200312

Informations de publication

Date de publication:
Apr 2022
Historique:
received: 27 01 2021
accepted: 17 06 2021
pubmed: 29 6 2021
medline: 21 5 2022
entrez: 28 6 2021
Statut: ppublish

Résumé

Cardiovascular disease is the major cause of death worldwide. Extensive cardiovascular biomarkers are available using blood tests but very few, if any, investigations have described non-invasive tests for cardiovascular biomarkers based on readily available hair samples. Here we show, first, that human hair proteins are post-translationally modified by arginine methylation (ArgMe). Using western blot, proteomic data mining and mass spectrometry, we identify several ArgMe events in hair proteins and we show that keratin-83 is extensively modified by ArgMe in the human hair. Second, using a preliminary cohort (n = 18) of heterogenous healthy donors, we show that the levels of protein ArgMe in hair correlate with serum concentrations of a well-established cardiovascular biomarker, asymmetric dimethylarginine (ADMA). Compared to blood collection, hair sampling is cheaper, simpler, requires minimal training and carries less health and safety and ethical risks. For these reasons, developing the potential of hair protein ArgMe as clinically useful cardiovascular biomarkers through further research could be useful in future prevention and diagnosis of cardiovascular disease.

Identifiants

pubmed: 34181092
doi: 10.1007/s00726-021-03024-5
pii: 10.1007/s00726-021-03024-5
pmc: PMC9117359
doi:

Substances chimiques

Biomarkers 0
Arginine 94ZLA3W45F

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

591-600

Subventions

Organisme : University of Hull (GB)
ID : Biomarkers Research Cluster
Organisme : Higher Education Funding Council for England
ID : HEIF

Informations de copyright

© 2021. The Author(s).

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Auteurs

Alistair James Marsden (AJ)

Department of Biomedical Sciences, University of Hull, Cottingham Rd, Hull, HU6 7RX, UK.

David R J Riley (DRJ)

Department of Biomedical Sciences, University of Hull, Cottingham Rd, Hull, HU6 7RX, UK.

Stefan Birkett (S)

Department of Sport, Health and Exercise Science, University of Hull, Hull, UK.
School of Sport and Health Sciences, University of Central Lancashire, Preston, UK.

Quentin Rodriguez-Barucg (Q)

Department of Biomedical Sciences, University of Hull, Cottingham Rd, Hull, HU6 7RX, UK.

Barbara-Ann Guinn (BA)

Department of Biomedical Sciences, University of Hull, Cottingham Rd, Hull, HU6 7RX, UK.

Sean Carroll (S)

Department of Sport, Health and Exercise Science, University of Hull, Hull, UK.

Lee Ingle (L)

Department of Sport, Health and Exercise Science, University of Hull, Hull, UK.

Thozhukat Sathyapalan (T)

Academic Endocrinology, Diabetes and Metabolism, Hull York Medical School, University of Hull, Hull, UK.

Pedro Beltran-Alvarez (P)

Department of Biomedical Sciences, University of Hull, Cottingham Rd, Hull, HU6 7RX, UK. p.beltran-alvarez@hull.ac.uk.

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