Coordinated β-glucosidase activity with the cellulosome is effective for enhanced lignocellulose saccharification.

Bacterial Proteins Cellulosomes Clostridium thermocellum Lignin beta-Glucosidase
Cellulosome Clostridium thermocellum Lignocellulose Saccharification β-glucosidase

Journal

Bioresource technology
ISSN: 1873-2976
Titre abrégé: Bioresour Technol
Pays: England
ID NLM: 9889523

Informations de publication

Date de publication:
Oct 2021
Historique:
received: 17 05 2021
revised: 16 06 2021
accepted: 17 06 2021
pubmed: 29 6 2021
medline: 29 7 2021
entrez: 28 6 2021
Statut: ppublish

Résumé

Consolidated bio-saccharification (CBS) technology employs cellulosome-producing bacterial cells, rather than fungal cellulases, as biocatalysts for cost-effective production of lignocellulosic sugars. Extracellular β-glucosidase (BGL) expression in the whole-cell arsenal is indispensable, due to severe cellobiose inhibition of the cellulosome. However, high-level BGL expression in Clostridium thermocellum is challenging, and the optimal BGL production level for efficient cellulose saccharification is currently unknown. Herein, we obtained new CBS biocatalysts by transforming BGL-expressing plasmids into C. thermocellum, which produced abundant BGL proteins and hydrolyzed cellulose effectively. The optimal ratio of extracellular BGL-to-cellulosome activity was determined to be in a range of 5.5 to 21.6. Despite the critical impact of BGL, both excessive BGL expression and its assembly on the cellulosome via type I cohesin-dockerin interaction led to reduced cellulosomal activity, which further confirmed the importance of coordinated BGL expression with the cellulosome. This study will further promote industrial CBS application in lignocellulose conversion.

Identifiants

DOI: 10.1016/j.biortech.2021.125441 PMID: 34182347
pubmed: 34182347
pii: S0960-8524(21)00781-1
doi: 10.1016/j.biortech.2021.125441
pii:
doi:

Substances chimiques

Bacterial Proteins 0
lignocellulose 11132-73-3
Lignin 9005-53-2
beta-Glucosidase EC 3.2.1.21

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

125441

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Auteurs

Kuan Qi (K)

CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Shandong Engineering Laboratory for Single Cell Oil, Qingdao Engineering Laboratory for Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Dalian National Laboratory for Clean Energy, Qingdao 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.

Chao Chen (C)

CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Shandong Engineering Laboratory for Single Cell Oil, Qingdao Engineering Laboratory for Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Dalian National Laboratory for Clean Energy, Qingdao 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.

Fei Yan (F)

CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Shandong Engineering Laboratory for Single Cell Oil, Qingdao Engineering Laboratory for Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Dalian National Laboratory for Clean Energy, Qingdao 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.

Yingang Feng (Y)

CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Shandong Engineering Laboratory for Single Cell Oil, Qingdao Engineering Laboratory for Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Dalian National Laboratory for Clean Energy, Qingdao 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.

Edward A Bayer (EA)

Department of Biomolecular Sciences, The Weizmann Institute of Science, Rehovot, Israel; Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva 8499000, Israel.

Akihiko Kosugi (A)

Biological Resources and Post-Harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan.

Qiu Cui (Q)

CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Shandong Engineering Laboratory for Single Cell Oil, Qingdao Engineering Laboratory for Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Dalian National Laboratory for Clean Energy, Qingdao 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.

Ya-Jun Liu (YJ)

CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Shandong Engineering Laboratory for Single Cell Oil, Qingdao Engineering Laboratory for Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Dalian National Laboratory for Clean Energy, Qingdao 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China. Electronic address: liuyj@qibebt.ac.cn.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Coordinated β-glucosidase activity with the...
questionsmedicales.fr › Cellules › Structures cellulaires › Prolongements cytoplasmiques › Cellulosomes › Coordinated β-glucosidase activity with the...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bactéries à Gram positif formant des endospores › Clostridium › Clostridium thermocellum › Coordinated β-glucosidase activity with the...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Biopolymères › Lignine › Coordinated β-glucosidase activity with the...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Glucosidases › Cellulases › bêta-Glucosidase › Coordinated β-glucosidase activity with the...