Role of S100A4 in the Pathogenesis of Human Periapical Granulomas.
Apical periodontitis
S100A4
endothelial cells
inflammatory mediators
periapical granulomas
real-time polymerase chain reaction
Journal
In vivo (Athens, Greece)
ISSN: 1791-7549
Titre abrégé: In Vivo
Pays: Greece
ID NLM: 8806809
Informations de publication
Date de publication:
Historique:
received:
10
05
2021
revised:
22
05
2021
accepted:
24
05
2021
entrez:
28
6
2021
pubmed:
29
6
2021
medline:
1
7
2021
Statut:
ppublish
Résumé
S100A4 expression is associated with the pathology of chronic inflammatory diseases. In this study, we investigated the role of S100A4 and four inflammatory mediators (IL-1β, IκB, IL-10, and TNF-α) in human periapical granulomas (PGs). S100A4 expression in PGs obtained by apicoectomy was examined by immunohistochemistry. Further, the expression of S100A4 and four inflammatory mediators was compared between PGs and healthy gingival tissues (HGTs) using real-time PCR. In the PGs, S100A4 was found to be expressed in endothelial cells and fibroblasts. Furthermore, real-time PCR revealed that the expression of S100A4 and IL-1β in PGs was significantly higher than that in HGTs. Although a correlation between the expression of S100A4 and IκB or IL-10 was not detected, a positive correlation between the expression of S100A4 and IL-1β or TNF-α was observed. The expression of S100A4 correlates with the pathogenesis of PGs.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
S100A4 expression is associated with the pathology of chronic inflammatory diseases. In this study, we investigated the role of S100A4 and four inflammatory mediators (IL-1β, IκB, IL-10, and TNF-α) in human periapical granulomas (PGs).
MATERIALS AND METHODS
METHODS
S100A4 expression in PGs obtained by apicoectomy was examined by immunohistochemistry. Further, the expression of S100A4 and four inflammatory mediators was compared between PGs and healthy gingival tissues (HGTs) using real-time PCR.
RESULTS
RESULTS
In the PGs, S100A4 was found to be expressed in endothelial cells and fibroblasts. Furthermore, real-time PCR revealed that the expression of S100A4 and IL-1β in PGs was significantly higher than that in HGTs. Although a correlation between the expression of S100A4 and IκB or IL-10 was not detected, a positive correlation between the expression of S100A4 and IL-1β or TNF-α was observed.
CONCLUSION
CONCLUSIONS
The expression of S100A4 correlates with the pathogenesis of PGs.
Identifiants
pubmed: 34182485
pii: 35/4/2099
doi: 10.21873/invivo.12479
pmc: PMC8286529
doi:
Substances chimiques
Inflammation Mediators
0
S100 Calcium-Binding Protein A4
0
Tumor Necrosis Factor-alpha
0
S100A4 protein, human
142662-27-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2099-2106Informations de copyright
Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
Références
J Immunol. 2008 Oct 1;181(7):4666-75
pubmed: 18802069
Rheumatology (Oxford). 2014 Aug;53(8):1520-6
pubmed: 24643522
Amino Acids. 2011 Oct;41(4):755-60
pubmed: 21120552
Amino Acids. 2011 Oct;41(4):863-73
pubmed: 20177948
Biochim Biophys Acta. 2013 Dec;1833(12):2653-2663
pubmed: 23830916
BMC Cancer. 2015 Feb 12;15:44
pubmed: 25884510
Biochim Biophys Acta Mol Cell Res. 2020 Jun;1867(6):118677
pubmed: 32057918
Ann Rheum Dis. 2008 Nov;67(11):1499-504
pubmed: 18056757
Int Endod J. 2006 Apr;39(4):249-81
pubmed: 16584489
BMB Rep. 2017 Feb;50(2):97-102
pubmed: 27998393
J Endod. 1992 Sep;18(9):422-6
pubmed: 9796508
Rheumatology (Oxford). 2009 Dec;48(12):1590-4
pubmed: 19828600
Crit Rev Oral Biol Med. 2004 Nov 01;15(6):348-81
pubmed: 15574679
Am J Pathol. 2010 Feb;176(2):528-35
pubmed: 20019188
Acta Pharmacol Sin. 2015 Nov;36(11):1388-94
pubmed: 26499072
Mol Biol (Mosk). 2016 Jul-Aug;50(4):577-588
pubmed: 27668598
Biochim Biophys Acta. 2009 Jun;1793(6):1008-22
pubmed: 19110011
Arch Oral Biol. 2015 Sep;60(9):1215-21
pubmed: 26079064
Curr Mol Med. 2013 Jan;13(1):24-57
pubmed: 22834835
J Endod. 1993 Mar;19(3):107-11
pubmed: 8509747
J Immunol. 2009 Jul 1;183(1):593-603
pubmed: 19542470
Immunol Rev. 2012 Mar;246(1):77-94
pubmed: 22435548
Eur J Surg Oncol. 2008 Apr;34(4):357-64
pubmed: 17566693
Oral Surg Oral Med Oral Pathol. 1965 Sep;20:340-9
pubmed: 14342926
J Endod. 2007 Aug;33(8):936-43
pubmed: 17878078
Adv Exp Med Biol. 1972;32(0):5-7
pubmed: 4604212
J Mol Cell Cardiol. 2009 Oct;47(4):445-55
pubmed: 19538970
Periodontol 2000. 1997 Feb;13:121-48
pubmed: 9567926
J Biol Chem. 2010 Oct 8;285(41):31517-24
pubmed: 20685652
Curr Top Microbiol Immunol. 2011;349:1-24
pubmed: 20845107
Adv Exp Med Biol. 2019;1172:207-226
pubmed: 31628658
Rheumatology (Oxford). 2011 Oct;50(10):1766-72
pubmed: 21712367