Multiscale mechanics and temporal evolution of vimentin intermediate filament networks.
cytoskeleton
intermediate filaments
microrheology
network mechanics
quadruple optical tweezers
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
06 07 2021
06 07 2021
Historique:
entrez:
30
6
2021
pubmed:
1
7
2021
medline:
15
12
2021
Statut:
ppublish
Résumé
The cytoskeleton, an intricate network of protein filaments, motor proteins, and cross-linkers, largely determines the mechanical properties of cells. Among the three filamentous components, F-actin, microtubules, and intermediate filaments (IFs), the IF network is by far the most extensible and resilient to stress. We present a multiscale approach to disentangle the three main contributions to vimentin IF network mechanics-single-filament mechanics, filament length, and interactions between filaments-including their temporal evolution. Combining particle tracking, quadruple optical trapping, and computational modeling, we derive quantitative information on the strength and kinetics of filament interactions. Specifically, we find that hydrophobic contributions to network mechanics enter mostly via filament-elongation kinetics, whereas electrostatics have a direct influence on filament-filament interactions.
Identifiants
pubmed: 34187892
pii: 2102026118
doi: 10.1073/pnas.2102026118
pmc: PMC8271578
pii:
doi:
Substances chimiques
Detergents
0
Ions
0
Vimentin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Déclaration de conflit d'intérêts
The authors declare no competing interest.
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