Contact-dependent inhibition of HIV-1 replication in
HIV-1 replication
antiviral activity
human tonsil explant culture
polymorphonuclear neutrophils
Journal
Cell reports. Medicine
ISSN: 2666-3791
Titre abrégé: Cell Rep Med
Pays: United States
ID NLM: 101766894
Informations de publication
Date de publication:
15 06 2021
15 06 2021
Historique:
received:
24
07
2020
revised:
02
03
2021
accepted:
20
05
2021
entrez:
1
7
2021
pubmed:
2
7
2021
medline:
2
7
2021
Statut:
epublish
Résumé
Polymorphonuclear neutrophils (PMNs), the most abundant white blood cells, are recruited rapidly to sites of infection to exert potent anti-microbial activity. Information regarding their role in infection with human immunodeficiency virus (HIV) is limited. Here we report that addition of PMNs to HIV-infected cultures of human tonsil tissue or peripheral blood mononuclear cells causes immediate and long-lasting suppression of HIV-1 spread and virus-induced depletion of CD4 T cells. This inhibition of HIV-1 spread strictly requires PMN contact with infected cells and is not mediated by soluble factors. 2-Photon (2PM) imaging visualized contacts of PMNs with HIV-1-infected CD4 T cells in tonsil tissue that do not result in lysis or uptake of infected cells. The anti-HIV activity of PMNs also does not involve degranulation, formation of neutrophil extracellular traps, or integrin-dependent cell communication. These results reveal that PMNs efficiently blunt HIV-1 replication in primary target cells and tissue by an unconventional mechanism.
Identifiants
pubmed: 34195682
doi: 10.1016/j.xcrm.2021.100317
pii: S2666-3791(21)00160-9
pmc: PMC8233696
doi:
Substances chimiques
Integrins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
100317Informations de copyright
© 2021 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests.
Références
Nat Med. 1995 Dec;1(12):1320-2
pubmed: 7489416
BioDrugs. 2013 Oct;27(5):479-93
pubmed: 23649937
J Virol. 1986 Dec;60(3):1068-74
pubmed: 3023659
J Immunol Methods. 1980;36(2):109-17
pubmed: 7430646
J Immunol. 1994 Apr 1;152(7):3361-9
pubmed: 7511644
Trends Microbiol. 2015 May;23(5):289-95
pubmed: 25766144
EMBO J. 2007 Jan 24;26(2):516-26
pubmed: 17215865
Cell Rep. 2018 Jun 26;23(13):3946-3959.e6
pubmed: 29949776
J Clin Invest. 1976 Sep;58(3):705-15
pubmed: 956397
Cell Host Microbe. 2012 Jul 19;12(1):109-16
pubmed: 22817992
Nat Commun. 2019 May 13;10(1):2144
pubmed: 31086185
EMBO J. 2020 Dec 15;39(24):e105594
pubmed: 33146906
Sci Immunol. 2018 Dec 7;3(30):
pubmed: 30530726
Scand J Immunol. 2017 Sep;86(3):143-155
pubmed: 28671713
Curr Opin HIV AIDS. 2015 Mar;10(2):96-102
pubmed: 25485569
Hum Pathol. 1988 Jul;19(7):849-54
pubmed: 2900210
Nat Rev Immunol. 2008 Sep;8(9):713-25
pubmed: 19172692
Arch Virol. 2018 Jan;163(1):1-21
pubmed: 28980078
J Acquir Immune Defic Syndr. 2006 Jan 1;41(1):6-16
pubmed: 16340467
Front Immunol. 2017 May 12;8:550
pubmed: 28553293
Antimicrob Agents Chemother. 2002 Jun;46(6):1896-905
pubmed: 12019106
Nat Rev Immunol. 2013 Mar;13(3):159-75
pubmed: 23435331
J Innate Immun. 2014;6(5):639-49
pubmed: 24713863
Nature. 2008 May 1;453(7191):51-5
pubmed: 18451854
Nat Med. 2021 Jan;27(1):125-135
pubmed: 33432170
mBio. 2013 Oct 29;4(6):e00810-13
pubmed: 24169578
PLoS One. 2013 Aug 30;8(8):e73659
pubmed: 24023689
Clin Exp Immunol. 2007 Dec;150(3):539-45
pubmed: 17956581
Cells. 2020 Jun 02;9(6):
pubmed: 32498376
Virology. 2006 Aug 1;351(2):322-39
pubmed: 16684552
J Clin Invest. 1992 Jun;89(6):2014-7
pubmed: 1318327
J Virol. 2008 Apr;82(8):3952-70
pubmed: 18256145
Am Rev Respir Dis. 1990 Jan;141(1):179-85
pubmed: 2297176
Nat Protoc. 2009;4(2):256-69
pubmed: 19197269
J Virol. 1998 Apr;72(4):2855-64
pubmed: 9525605
Curr Opin Hematol. 2010 Jan;17(1):9-17
pubmed: 19864945
Clin Infect Dis. 2011 May;52(10):1248-56
pubmed: 21507922
J Virol. 2005 Apr;79(8):5220-6
pubmed: 15795306
Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):663-8
pubmed: 9892690
J Med Virol. 2020 Nov;92(11):2573-2581
pubmed: 32458459
Biochem Pharmacol. 2000 Sep 1;60(5):729-33
pubmed: 10927032
Thromb Res. 2020 Jul;191:26-27
pubmed: 32360977
J Transl Med. 2020 May 20;18(1):206
pubmed: 32434518
J Virol. 2019 Dec 12;94(1):
pubmed: 31597760
Infect Immun. 2002 Jul;70(7):3363-70
pubmed: 12065474
Immunity. 2001 Oct;15(4):671-82
pubmed: 11672548
Science. 2004 Mar 5;303(5663):1532-5
pubmed: 15001782
Biol Chem. 2008 Oct;389(10):1253-60
pubmed: 18713012
J Gen Virol. 2013 Jan;94(Pt 1):40-49
pubmed: 23052388
Nat Med. 2012 Nov;18(11):1682-7
pubmed: 22972397
N Engl J Med. 2012 Dec 6;367(23):2260-2
pubmed: 23215575
Respir Res. 2008 Aug 01;9:57
pubmed: 18671884
J Leukoc Biol. 2015 Oct;98(4):557-64
pubmed: 26160849
Nat Rev Immunol. 2011 Jul 25;11(8):519-31
pubmed: 21785456
J Exp Med. 2006 Nov 27;203(12):2569-75
pubmed: 17116736
J Exp Med. 2013 Jul 1;210(7):1283-99
pubmed: 23825232
Arch Virol. 1989;104(3-4):287-98
pubmed: 2539796
Proc Natl Acad Sci U S A. 2008 May 27;105(21):7552-7
pubmed: 18490657
J Virol. 2007 Nov;81(22):12582-95
pubmed: 17728240
Cells. 2020 Jan 20;9(1):
pubmed: 31968566
PLoS Biol. 2018 Feb 6;16(2):e2003885
pubmed: 29408891
J Immunol Methods. 2016 Nov;438:35-41
pubmed: 27589923
J Clin Invest. 2012 Jan;122(1):327-36
pubmed: 22156198
Cold Spring Harb Perspect Med. 2012 May;2(5):a006940
pubmed: 22553496
Cell Tissue Res. 2018 Mar;371(3):505-516
pubmed: 29327081
Cells. 2020 Apr 30;9(5):
pubmed: 32365826
PLoS One. 2013 Sep 09;8(9):e72034
pubmed: 24039734
J Virol. 2005 Dec;79(23):14933-44
pubmed: 16282492
J Virol. 1999 Aug;73(8):6380-6
pubmed: 10400730
Cell Rep. 2016 Oct 4;17(2):413-424
pubmed: 27705790
Mucosal Immunol. 2018 Sep;11(5):1420-1428
pubmed: 29875403
Retrovirology. 2009 Jan 15;6:6
pubmed: 19146681
Immunity. 2016 Feb 16;44(2):233-45
pubmed: 26872694
Nat Rev Immunol. 2006 Jun;6(6):447-56
pubmed: 16724099
J Virol. 2004 Oct;78(19):10833-6
pubmed: 15367652
Blood. 2010 Jul 29;116(4):625-7
pubmed: 20410504
Ther Clin Risk Manag. 2019 Jul 24;15:911-919
pubmed: 31413580
Blood. 2001 Apr 15;97(8):2478-86
pubmed: 11290613
Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8392-6
pubmed: 7690960