Identification and Functional Characterization of Viroid Circular RNAs.
Infectious RNA
Noncoding RNA
Plant circular RNA
RNA circularization
RNA replication
Ribozyme
Viroid
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
1
7
2021
pubmed:
2
7
2021
medline:
13
1
2022
Statut:
ppublish
Résumé
Viroids are relatively small, noncoding, plant circular RNAs. In contrast to other plant circular RNAs of endogenous origin, viroids are infectious agents able to replicate autonomously in the appropriate host. Because of their highly base-paired structures, they can be purified from infected tissue extracts using nonionic CF11 chromatography. Depending on the host plant species, viroid RNA preparation may also require polysaccharide removal by an extraction with 2-methoxyethanol followed by precipitation with cetyltrimethylammonium bromide. Electrophoretic analyses of this kind of preparations frequently show differential bands corresponding to the viroid circular molecules, which are absent in those from healthy plants. These RNA preparations can also be used for viroid transmission to new plants by mechanical inoculation.
Identifiants
pubmed: 34195959
doi: 10.1007/978-1-0716-1645-1_6
doi:
Substances chimiques
RNA, Circular
0
RNA, Viral
0
RNA
63231-63-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101-107Références
Haddad G, Lorenzen JM (2019) Biogenesis and function of circular RNAs in health and in disease. Front Pharmacol 10:428. https://doi.org/10.3389/fphar.2019.00428
doi: 10.3389/fphar.2019.00428
pubmed: 31080413
pmcid: 6497739
Diener TO (1971) Potato spindle tuber “virus” IV. A replicating, low molecular weight RNA. Virology 45:411–428
doi: 10.1016/0042-6822(71)90342-4
Sänger HL, Klotz G, Riesner D et al (1976) Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures. Proc Natl Acad Sci U S A 73:3852–3856. https://doi.org/10.1073/pnas.73.11.3852
doi: 10.1073/pnas.73.11.3852
pubmed: 1069269
pmcid: 431239
Di Serio F, Flores R, Verhoeven JT et al (2014) Current status of viroid taxonomy. Arch Virol 159:3467–3478. https://doi.org/10.1007/s00705-014-2200-6
doi: 10.1007/s00705-014-2200-6
pubmed: 25216773
Elena SF, Dopazo J, de la Pena M et al (2001) Phylogenetic analysis of viroid and viroid-like satellite RNAs from plants: a reassessment. J Mol Evol 53:155–159
doi: 10.1007/s002390010203
Branch AD, Robertson HD (1984) A replication cycle for viroids and other small infectious RNAs. Science 223:450–455
doi: 10.1126/science.6197756
Gas M-E, Molina-Serrano D, Hernández C et al (2008) Monomeric linear RNA of citrus Exocortis viroid resulting from processing in vivo has 5′-phosphomonoester and 3′-hydroxyl termini: implications for the RNase and RNA ligase involved in replication. J Virol 82:10321–10325. https://doi.org/10.1128/JVI.01229-08
doi: 10.1128/JVI.01229-08
pubmed: 18701598
pmcid: 2566280
Nohales MÁ, Flores R, Daròs JA (2012) Viroid RNA redirects host DNA ligase 1 to act as an RNA ligase. Proc Natl Acad Sci U S A 109:13805–13810. https://doi.org/10.1073/pnas.1206187109
doi: 10.1073/pnas.1206187109
pubmed: 22869737
pmcid: 3427106
Nohales M-A, Molina-Serrano D, Flores R et al (2012) Involvement of the Chloroplastic isoform of tRNA ligase in the replication of Viroids belonging to the family Avsunviroidae. J Virol 86:8269–8276. https://doi.org/10.1128/jvi.00629-12
doi: 10.1128/jvi.00629-12
pubmed: 22623792
pmcid: 3421689
Daròs JA (2016) Eggplant latent viroid: a friendly experimental system in the family Avsunviroidae. Mol Plant Pathol 17:1170–1177
doi: 10.1111/mpp.12358
Navarro B, Flores R (1997) Chrysanthemum chlorotic mottle viroid: unusual structural properties of a subgroup of self-cleaving viroids with hammerhead ribozymes. Proc Natl Acad Sci U S A 94:11262–11267
doi: 10.1073/pnas.94.21.11262
Franklin RM (1966) Purification and propertiels of replicative intermediate of RNA bacteriophage R17. Proc Natl Acad Sci U S A 55:1504
doi: 10.1073/pnas.55.6.1504
Daròs JA (2021) Two-dimensional polyacrylamide-gel electrophoresis analysis of viroid RNAs. In: Vidalakis G et al. (eds) Viroids: methods and protocols. Methods molecular biology, vol 2316. Springer US, New York
Bellamy AR, Ralph RK (1968) Recovery and purification of nucleic acids by means of cetyltrimethylammonium bromide. Methods Enzymol 12:156–160. https://doi.org/10.1016/0076-6879(67)12125-3
doi: 10.1016/0076-6879(67)12125-3