Development of a Monocyte Activation Test as an Alternative to the Rabbit Pyrogen Test for Mono- and Multi-Component
generalised modules for membrane antigens (GMMA)
monocyte activation test (MAT)
outer membrane vesicle (OMV)
pyrogenicity
reactogenicity
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
24 Jun 2021
24 Jun 2021
Historique:
received:
21
05
2021
revised:
14
06
2021
accepted:
15
06
2021
entrez:
2
7
2021
pubmed:
3
7
2021
medline:
3
7
2021
Statut:
epublish
Résumé
Generalised modules for membrane antigens (GMMA)-based vaccines comprise the outer membrane from genetically modified Gram-negative bacteria containing membrane proteins, phospholipids and lipopolysaccharides. Some lipoproteins and lipopolysaccharides are pyrogens; thus, GMMA-based vaccines are intrinsically pyrogenic. It is important to control the pyrogenic content of biological medicines, including vaccines, to prevent adverse reactions such as febrile responses. The rabbit pyrogen test (RPT) and bacterial endotoxin test (BET) are the most commonly employed safety assays used to detect pyrogens. However, both tests are tailored for detecting pyrogenic contaminants and have considerable limitations when measuring the pyrogen content of inherently pyrogenic products. We report the adaptation of the monocyte activation test (MAT) as an alternative to the RPT for monitoring the pyrogenicity of
Identifiants
pubmed: 34202832
pii: microorganisms9071375
doi: 10.3390/microorganisms9071375
pmc: PMC8306433
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : UK Department of Health and Social Care
ID : EP/R013764/1
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