Novel mumps virus epitopes reveal robust cytotoxic T cell responses after natural infection but not after vaccination.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
01 07 2021
Historique:
received: 14 01 2021
accepted: 18 06 2021
entrez: 2 7 2021
pubmed: 3 7 2021
medline: 9 11 2021
Statut: epublish

Résumé

Mumps is nowadays re-emerging despite vaccination. The contribution of T cell immunity to protection against mumps has not been clearly defined. Previously, we described a set of 41 peptides that were eluted from human leukocyte antigen (HLA) class I molecules of mumps virus (MuV)-infected cells. Here, we confirmed immunogenicity of five novel HLA-B*07:02- and HLA-A*01:01-restricted MuV T cell epitopes from this set of peptides. High frequencies of T cells against these five MuV epitopes could be detected ex vivo in all tested mumps patients. Moreover, these epitope-specific T cells derived from mumps patients displayed strong cytotoxic activity. In contrast, only marginal T cell responses against these novel MuV epitopes could be detected in recently vaccinated persons, corroborating earlier findings. Identifying which MuV epitopes are dominantly targeted in the mumps-specific CD8

Identifiants

pubmed: 34211021
doi: 10.1038/s41598-021-92926-1
pii: 10.1038/s41598-021-92926-1
pmc: PMC8249633
doi:

Substances chimiques

Epitopes, T-Lymphocyte 0
HLA-A Antigens 0
HLA-B Antigens 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

13664

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Auteurs

Patricia Kaaijk (P)

Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), PO box 41, 3720, BA, Bilthoven, The Netherlands. patricia.kaaijk@rivm.nl.

Maarten E Emmelot (ME)

Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), PO box 41, 3720, BA, Bilthoven, The Netherlands.

Hugo D Meiring (HD)

Intravacc (Institute for Translational Vaccinology), Bilthoven, The Netherlands.

Cécile A C M van Els (CACM)

Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), PO box 41, 3720, BA, Bilthoven, The Netherlands.

Jelle de Wit (J)

Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), PO box 41, 3720, BA, Bilthoven, The Netherlands.

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Classifications MeSH