A novel platform for drug testing: Biomimetic three-dimensional hyaluronic acid-based scaffold seeded with human hepatocarcinoma cells.
Biomarkers, Tumor
/ metabolism
Biomimetic Materials
/ chemistry
Carcinoma, Hepatocellular
/ drug therapy
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
Cholesterol 7-alpha-Hydroxylase
/ metabolism
Cisplatin
/ chemistry
Drug Screening Assays, Antitumor
Gene Expression Regulation, Neoplastic
/ drug effects
Hep G2 Cells
Humans
Hyaluronic Acid
/ chemistry
Liver Neoplasms
/ drug therapy
Tissue Scaffolds
3D cell culture
Cisplatin
Drug testing
HepG2 cells
Hyaluronic acid-based scaffold
Tumor model
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
31 Aug 2021
31 Aug 2021
Historique:
received:
27
04
2021
revised:
09
06
2021
accepted:
26
06
2021
pubmed:
4
7
2021
medline:
19
8
2021
entrez:
3
7
2021
Statut:
ppublish
Résumé
Hepatic cancer is one of the most widespread maladies worldwide that requires urgent therapies and thus reliable means for testing anti-cancer drugs. The switch from two-dimensional (2D) to three-dimensional (3D) cell cultures produced an improvement in the in vitro outcomes for testing anti-cancer drugs. We aimed to develop a novel hyaluronic acid (HA)-based 3D cell model of human hepatocellular carcinoma (HepG2 cells) for drug testing and to assess comparatively in 3D vs. 2D, the cytotoxicity and the apoptotic response to the anti-tumor agent, cisplatin. The 3D model was developed by seeding HepG2 cells in a HA/poly(methylvinylether-alt-maleic acid) (HA
Identifiants
pubmed: 34216662
pii: S0141-8130(21)01403-3
doi: 10.1016/j.ijbiomac.2021.06.174
pii:
doi:
Substances chimiques
Biomarkers, Tumor
0
Hyaluronic Acid
9004-61-9
CYP7A1 protein, human
EC 1.14.14.23
Cholesterol 7-alpha-Hydroxylase
EC 1.14.14.23
Cisplatin
Q20Q21Q62J
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
604-619Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.