Immunocytochemical evaluation of epithelial-mesenchymal transition in epithelial tumors of dogs and cats.
E-cadherin
cytology
epithelial-mesenchymal transition
immunocytochemistry
vimentin
Journal
The Journal of veterinary medical science
ISSN: 1347-7439
Titre abrégé: J Vet Med Sci
Pays: Japan
ID NLM: 9105360
Informations de publication
Date de publication:
03 Sep 2021
03 Sep 2021
Historique:
pubmed:
9
7
2021
medline:
8
9
2021
entrez:
8
7
2021
Statut:
ppublish
Résumé
Epithelial-mesenchymal transition (EMT) plays a crucial role in metastasis of epithelial tumors; however, it is challenging to detect EMT by cytology. In the present study, EMT was visualized by fluorescence-immunocytochemistry (FICC). Air-dried smears from epithelial tumors of dogs (n=22) and cats (n=9) were stained using mouse monoclonal anti-E-cadherin and rabbit monoclonal anti-vimentin antibodies. Enzymatic immunohistochemistry (IHC) revealed that 51.6% (8/22 in dogs, 8/9 in cats) of the cases showed EMT. In dogs, FICC could detect EMT in 62.5% (5/8) of those cases. In cats, FICC could detect EMT in 100% (8/8) of the cases. In conclusion, the present FICC method could successfully detect EMT using conventional air-dried cytology smear slides.
Identifiants
pubmed: 34234057
doi: 10.1292/jvms.21-0021
pmc: PMC8498836
doi:
Substances chimiques
Vimentin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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