Systemic treatment with 7,8-Dihydroxiflavone activates TtkB and affords protection of two different retinal ganglion cell populations against axotomy in adult rats.
Animals
Axotomy
Blotting, Western
Cell Survival
/ physiology
Female
Flavones
/ administration & dosage
Immunohistochemistry
Injections, Intraperitoneal
Neuroprotection
Neuroprotective Agents
/ administration & dosage
Optic Nerve
/ physiopathology
Phosphorylation
Rats
Rats, Sprague-Dawley
Receptor, trkB
/ metabolism
Retinal Ganglion Cells
/ drug effects
Rod Opsins
/ metabolism
Transcription Factor Brn-3A
/ metabolism
7,8-Dihydroxiflavone
Adult albino rats
Apoptosis
Axotomy
BDNF neuroprotection
BDNF-Mimetic
Brn3a
Intraorbital optic nerve transection
Intrinsically photosensitive retinal ganglion cells
Melanopsin
Neuroprotection
Optic nerve section
Retinal ganglion cells
Journal
Experimental eye research
ISSN: 1096-0007
Titre abrégé: Exp Eye Res
Pays: England
ID NLM: 0370707
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
received:
01
04
2021
revised:
26
05
2021
accepted:
01
07
2021
pubmed:
11
7
2021
medline:
9
10
2021
entrez:
10
7
2021
Statut:
ppublish
Résumé
To analyze responses of different RGC populations to left intraorbital optic nerve transection (IONT) and intraperitoneal (i.p.) treatment with 7,8-Dihydroxyflavone (DHF), a potent selective TrkB agonist. Adult albino Sprague-Dawley rats received, following IONT, daily i.p. injections of vehicle (1%DMSO in 0.9%NaCl) or DHF. Group-1 (n = 58) assessed at 7days (d) the optimal DHF amount (1-25 mg/kg). Group-2, using freshly dissected naïve or treated retinas (n = 28), investigated if DHF treatment was associated with TrkB activation using Western-blotting at 1, 3 or 7d. Group-3 (n = 98) explored persistence of protection and was analyzed at survival intervals from 7 to 60d after IONT. Groups 2-3 received daily i.p. vehicle or DHF (5 mg/kg). Retinal wholemounts were immunolabelled for Brn3a and melanopsin to identify Brn3a Optimal neuroprotection was achieved with 5 mg/kg DHF and resulted in TrkB phosphorylation. The percentage of surviving Brn3a DHF neuroprotects Brn3a
Identifiants
pubmed: 34245756
pii: S0014-4835(21)00260-8
doi: 10.1016/j.exer.2021.108694
pii:
doi:
Substances chimiques
6,7-dihydroxyflavone
0
Flavones
0
Neuroprotective Agents
0
Rod Opsins
0
Transcription Factor Brn-3A
0
melanopsin
0
Ntrk2 protein, rat
EC 2.7.10.1
Receptor, trkB
EC 2.7.10.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108694Informations de copyright
Copyright © 2021. Published by Elsevier Ltd.