Lipid metabolism research in oleaginous fungus Mortierella alpina: Current progress and future prospects.

Arachidonic Acid / metabolism Lipid Metabolism Lipogenesis / genetics Mortierella / genetics
Genetic manipulation Lipid metabolism Long-chain polyunsaturated fatty acids Mortierella alpina Multi-omics study Multidimensional engineering Oleaginous microorganism

Journal

Biotechnology advances
ISSN: 1873-1899
Titre abrégé: Biotechnol Adv
Pays: England
ID NLM: 8403708

Informations de publication

Date de publication:
Historique:
received: 27 02 2021
revised: 11 06 2021
accepted: 04 07 2021
pubmed: 11 7 2021
medline: 22 3 2022
entrez: 10 7 2021
Statut: ppublish

Résumé

The oleaginous fungus Mortierella alpina has distinct advantages in long-chain PUFAs production, and it is the only source for dietary arachidonic acid (ARA) certificated by FDA and European Commission. This review provides an overall introduction to M. alpina, including its major research methods, key factors governing lipid biosynthesis, metabolic engineering and omics studies. Currently, the research interests in M. alpina focus on improving lipid yield and fatty acid desaturation degree by enhancing fatty acid precursors and the reducing power NADPH, and genetic manipulation on PUFAs synthetic pathways is carried to optimise fatty acid composition. Besides, multi-omics studies have been applied to elucidate the global regulatory mechanism of lipogenesis in M. alpina. However, research challenges towards achieving a lipid cell factory lie in strain breeding and cost control due to the coenocytic mycelium, long fermentation period and insufficient conversion rate from carbon to lipid. We also proposed future research goals based on a multilevel regulating strategy: obtaining ideal chassis by directional evolution and high-throughput screening; rewiring central carbon metabolism and inhibiting competitive pathways by multi-gene manipulation system to enhance carbon to lipid conversion rate; optimisation of protein function based on post-translational modification; application of dynamic fermentation strategies suitable for different fermentation phases. By reviewing the comprehensive research progress of this oleaginous fungus, we aim to further comprehend the fungal lipid metabolism and provide reference information and guidelines for the exploration of microbial oils from the perspectives of fundamental research to industrial application.

Identifiants

DOI: 10.1016/j.biotechadv.2021.107794 PMID: 34245810
pubmed: 34245810
pii: S0734-9750(21)00100-2
doi: 10.1016/j.biotechadv.2021.107794
pii:
doi:

Substances chimiques

Arachidonic Acid 27YG812J1I

Types de publication

Journal Article Research Support, Non-U.S. Gov't Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

107794

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Auteurs

Lulu Chang (L)

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China. Electronic address: 7180112002@stu.jiangnan.edu.cn.

Hengqian Lu (H)

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China. Electronic address: hengqianlu@163.com.

Haiqin Chen (H)

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China. Electronic address: haiqinchen@jiangnan.edu.cn.

Xin Tang (X)

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China. Electronic address: xintang@jiangnan.edu.cn.

Jianxin Zhao (J)

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China. Electronic address: zhaojianxin@jiangnan.edu.cn.

Hao Zhang (H)

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China. Electronic address: zhanghao@jiangnan.edu.cn.

Yong Q Chen (YQ)

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu 214122, PR China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu 214122, PR China; Wuxi Translational Medicine Research Center, Jiangsu Translational Medicine Research Institute Wuxi Branch, Wuxi, Jiangsu 214122, PR China; Department of Cancer Biology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA. Electronic address: yqchen@jiangnan.edu.cn.

Wei Chen (W)

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu 214122, PR China. Electronic address: chenwei66@jiangnan.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Lipides › Acides gras › Acides gras insaturés › Acides gras indispensables › Acides arachidoniques › Acide arachidonique › Lipid metabolism research in oleaginous fungus...
questionsmedicales.fr › Métabolisme › Métabolisme lipidique › Lipid metabolism research in oleaginous fungus...
questionsmedicales.fr › Métabolisme › Métabolisme lipidique › Lipogenèse › Lipid metabolism research in oleaginous fungus...
questionsmedicales.fr › Eucaryotes › Champignons › Champignons non classés › Mucorales › Mortierella › Lipid metabolism research in oleaginous fungus...