The inhibitory receptor CD300a is essential for neutrophil-mediated clearance of urinary tract infection in mice.
Animals
Apoptosis
/ immunology
Escherichia coli Infections
/ immunology
Escherichia coli Proteins
/ metabolism
Female
Gene Knockout Techniques
Hemolysin Proteins
/ metabolism
Mice
Mice, Inbred BALB C
Neutrophils
/ immunology
Phagocytosis
/ genetics
Phosphatidylethanolamines
/ metabolism
Phosphatidylserines
/ metabolism
Receptors, Immunologic
/ deficiency
Urinary Bladder
/ immunology
Urinary Tract Infections
/ immunology
Uropathogenic Escherichia coli
/ growth & development
CD300a
Epithelial cells
Neutrophils
Urinary tract infection
α-hemolysin
Journal
European journal of immunology
ISSN: 1521-4141
Titre abrégé: Eur J Immunol
Pays: Germany
ID NLM: 1273201
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
revised:
18
05
2021
received:
05
10
2020
accepted:
12
07
2021
pubmed:
17
7
2021
medline:
17
11
2021
entrez:
16
7
2021
Statut:
ppublish
Résumé
Neutrophils play a crucial role in immune defense against and clearance of uropathogenic Escherichia coli (UPEC)-mediated urinary tract infection, the most common bacterial infection in healthy humans. CD300a is an inhibitory receptor that binds phosphatidylserine and phosphatidylethanolamine, presented on the membranes of apoptotic cells. CD300a binding to phosphatidylserine and phosphatidylethanolamine, also known as the "eat me" signal, mediates immune tolerance to dying cells. Here, we demonstrate for the first time that CD300a plays an important role in the neutrophil-mediated immune response to UPEC-induced urinary tract infection. We show that CD300a-deficient neutrophils have impaired phagocytic abilities and despite their increased accumulation at the site of infection, they are unable to reduce bacterial burden in the bladder, which results in significant exacerbation of infection and worse host outcome. Finally, we demonstrate that UPEC's pore forming toxin α-hemolysin induces upregulation of the CD300a ligand on infected bladder epithelial cells, signaling to neutrophils to be cleared.
Identifiants
pubmed: 34268737
doi: 10.1002/eji.202049006
doi:
Substances chimiques
Escherichia coli Proteins
0
Hemolysin Proteins
0
Hlya protein, E coli
0
LMIR1 protein, mouse
0
Phosphatidylethanolamines
0
Phosphatidylserines
0
Receptors, Immunologic
0
phosphatidylethanolamine
39382-08-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2218-2224Informations de copyright
© 2021 Wiley-VCH GmbH.
Références
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