Monitoring Human Neutrophil Activation by a Proteinase 3 Near-Infrared Fluorescence Substrate-Based Probe.

Cell Survival Fluorescent Dyes Gene Expression Regulation, Enzymologic / drug effects Humans Ionomycin Microscopy, Confocal Molecular Structure Myeloblastin / chemistry Neutrophil Activation / physiology Neutrophils / drug effects Spectrophotometry, Infrared

Journal

Bioconjugate chemistry
ISSN: 1520-4812
Titre abrégé: Bioconjug Chem
Pays: United States
ID NLM: 9010319

Informations de publication

Date de publication:
18 08 2021
Historique:
pubmed: 17 7 2021
medline: 15 12 2021
entrez: 16 7 2021
Statut: ppublish

Résumé

A near-infrared fluorescent (NIRF) substrate-based probe (SBP) was conceived to monitor secreted human proteinase 3 (hPR3) activity. This probe, called pro3-SBP, is shaped by a fused peptide hairpin loop structure, which associates a hPR3 recognition domain (Val-Ala-Asp-Nva-Ala-Asp-Tyr-Gln, where Nva is norvaline) and an electrostatic zipper (consisting of complementary polyanionic (d-Glu)

Identifiants

DOI: 10.1021/acs.bioconjchem.1c00267 PMID: 34269060
pubmed: 34269060
doi: 10.1021/acs.bioconjchem.1c00267
doi:

Substances chimiques

Fluorescent Dyes 0
Ionomycin 56092-81-0
Myeloblastin EC 3.4.21.76

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1782-1790

Auteurs

Ahlame Saidi (A)

Université de Tours, Tours 37032, France.
UMR 1100, Research Center for Respiratory Diseases (CEPR), Team: "Proteolytic Mechanisms in Inflammation", INSERM, Tours 37032, France.

Mylène Wartenberg (M)

Université de Tours, Tours 37032, France.
UMR 1100, Research Center for Respiratory Diseases (CEPR), Team: "Proteolytic Mechanisms in Inflammation", INSERM, Tours 37032, France.

Jean-Baptiste Madinier (JB)

Center for Molecular Biophysics (CBM), Team: "Molecular, Structural and Chemical Biology″, CNRS UPR 4301, Orléans 45071, France.

Guy Ilango (G)

IBiSA Electron Microscopy Platform, Université de Tours, Tours 37032, France.

Seda Seren (S)

Université de Tours, Tours 37032, France.
UMR 1100, Research Center for Respiratory Diseases (CEPR), Team: "Proteolytic Mechanisms in Inflammation", INSERM, Tours 37032, France.

Brice Korkmaz (B)

Université de Tours, Tours 37032, France.
UMR 1100, Research Center for Respiratory Diseases (CEPR), Team: "Proteolytic Mechanisms in Inflammation", INSERM, Tours 37032, France.
ORCID: 0000-0002-5159-8706

Fabien Lecaille (F)

Université de Tours, Tours 37032, France.
UMR 1100, Research Center for Respiratory Diseases (CEPR), Team: "Proteolytic Mechanisms in Inflammation", INSERM, Tours 37032, France.

Vincent Aucagne (V)

Center for Molecular Biophysics (CBM), Team: "Molecular, Structural and Chemical Biology″, CNRS UPR 4301, Orléans 45071, France.

Gilles Lalmanach (G)

Université de Tours, Tours 37032, France.
UMR 1100, Research Center for Respiratory Diseases (CEPR), Team: "Proteolytic Mechanisms in Inflammation", INSERM, Tours 37032, France.
ORCID: 0000-0001-8562-4821

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Survie cellulaire › Monitoring Human Neutrophil Activation by a...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › Monitoring Human Neutrophil Activation by a...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes codant pour des enzymes › Monitoring Human Neutrophil Activation by a...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Monitoring Human Neutrophil Activation by a...
questionsmedicales.fr › Lipides › Acides gras › Acides gras insaturés › Ionomycine › Monitoring Human Neutrophil Activation by a...
questionsmedicales.fr › Techniques d'investigation › Microscopie confocale › Monitoring Human Neutrophil Activation by a...
questionsmedicales.fr › Phénomènes chimiques › Structure moléculaire › Monitoring Human Neutrophil Activation by a...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Protéases à sérine › Serine endopeptidases › Myéloblastine › Monitoring Human Neutrophil Activation by a...
questionsmedicales.fr › Phénomènes du système immunitaire › Activation des neutrophiles › Monitoring Human Neutrophil Activation by a...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Granulocytes neutrophiles › Monitoring Human Neutrophil Activation by a...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectrophotométrie › Spectrophotométrie IR › Monitoring Human Neutrophil Activation by a...