The circulatory small non-coding RNA landscape in community-acquired pneumonia on intensive care unit admission.
Aged
Community-Acquired Infections
/ blood
Female
Humans
Intensive Care Units
/ organization & administration
Male
MicroRNAs
/ blood
Middle Aged
Pneumococcal Infections
/ blood
Pneumonia
/ blood
RNA, Small Untranslated
/ blood
Sepsis
/ blood
Severity of Illness Index
Streptococcus pneumoniae
/ genetics
Streptococcus pneumoniae
Sepsis
community-acquired pneumonia
micro RNA
small non-coding RNA
Journal
Journal of cellular and molecular medicine
ISSN: 1582-4934
Titre abrégé: J Cell Mol Med
Pays: England
ID NLM: 101083777
Informations de publication
Date de publication:
08 2021
08 2021
Historique:
revised:
10
02
2021
received:
05
12
2020
accepted:
12
02
2021
pubmed:
18
7
2021
medline:
19
2
2022
entrez:
17
7
2021
Statut:
ppublish
Résumé
Community-acquired pneumonia (CAP) is a major cause of sepsis. Despite several clinical trials targeting components of the inflammatory response, no specific treatment other than antimicrobial therapy has been approved. This argued for a deeper understanding of sepsis immunopathology, in particular factors that can modulate the host response. Small non-coding RNA, for example, micro (mi)RNA, have been established as important modifiers of cellular phenotypes. Notably, miRNAs are not exclusive to the intracellular milieu but have also been detected extracellular in the circulation with functional consequences. Here, we sought to determine shifts in circulatory small RNA levels of critically ill patients with CAP-associated sepsis and to determine the influence of clinical severity and causal pathogens on small RNA levels. Blood plasma was collected from 13 critically ill patients with sepsis caused by CAP on intensive care unit admission and from 5 non-infectious control participants. Plasma small RNA-sequencing identified significantly altered levels of primarily mature miRNAs in CAP relative to controls. Pathways analysis of high or low abundance miRNA identified various over-represented cellular biological pathways. Analysis of small RNA levels against common clinical severity and inflammatory parameters indices showed direct and indirect correlations. Additionally, variance of plasma small RNA levels in CAP patients may be explained, at least in part, by differences in causal pathogens. Small nuclear RNA levels were specifically altered in CAP due to Influenza infection in contrast to Streptococcus pneumoniae infection. Pathway analysis of plasma miRNA signatures unique to Influenza or Streptococcus pneumoniae infections showed enrichment for specific proteoglycan, cell cycle, and immunometabolic pathways.
Identifiants
pubmed: 34272809
doi: 10.1111/jcmm.16406
pmc: PMC8358855
doi:
Substances chimiques
MicroRNAs
0
RNA, Small Untranslated
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7621-7630Informations de copyright
© 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
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