Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China.
Borrelia miyamotoi
Inner Mongolia
Ixodes persulcatus
MLSA
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
17 Jul 2021
17 Jul 2021
Historique:
received:
24
02
2021
accepted:
25
05
2021
entrez:
18
7
2021
pubmed:
19
7
2021
medline:
20
11
2021
Statut:
epublish
Résumé
Borrelia miyamotoi is a newly described relapsing fever spirochete transmitted by ixodid tick species. Little is known about the prevalence of B. miyamotoi infections in humans and ticks in Inner Mongolia, China. Therefore, we investigated the prevalence of B. miyamotoi in Ixodes persulcatus ticks, and we aimed to isolateB. miyamotoi from I. persulcatus from four regions of Greater Khingan, Inner Mongolia, China. From May to June each year during the period 2016-2019, host-seeking adult I. persulcatus ticks were collected from vegetation. Genomic DNA was prepared from half of each tick body for PCR template, and the remaining half was used to cultivate B. miyamotoi in BSK-M medium. We employed quantitative real-time PCR (qPCR) to detect Borrelia DNA in the ticks and to calculate the prevalence of B. miyamotoi and infections with other borreliae. For characterization of the isolated B. miyamotoi, we performed draft genome sequencing and multilocus sequencing analysis (MLSA). A total of 2656 adult I. persulcatus ticks were collected. The overall prevalence of relapsing fever (RF) borreliae in ticks was 5.0% (134/2656) and that of Lyme disease (LD) borreliae was 43.8% (1164/2656). Co-infection with RF and LD borreliae was observed in 63 ticks (2.4%). Ticks that were positive for RF borreliae by qPCR were subjected to glycerophosphodiester diester phosphodiesterase gene (glpQ) PCR amplification and sequencing, through which we identified the RF borrelia specimens as B. miyamotoi. Furthermore, the B. miyamotoi strain Hetao-1 was isolated from I. persulcatus, and a draft genome sequence was obtained from the isolate. Sequencing determined the strain Hetao-1 genome to be approximately 906.1 kbp in length (28.9% average GC content), and MLSA identified the strain as ST633, which has previously been reported in Japan and Mongolia. We detected B. miyamotoi from I. persulcatus ticks collected in Inner Mongolia, and successfully isolated a B. miyamotoi strain. To our knowledge, this is the first study to culture a B. miyamotoi isolate from China. The data on the prevalence of B. miyamotoi and other borreliae in I. persulcatus ticks will be fundamental for future epidemiological studies of B. miyamotoi disease in Inner Mongolia.
Sections du résumé
BACKGROUND
BACKGROUND
Borrelia miyamotoi is a newly described relapsing fever spirochete transmitted by ixodid tick species. Little is known about the prevalence of B. miyamotoi infections in humans and ticks in Inner Mongolia, China. Therefore, we investigated the prevalence of B. miyamotoi in Ixodes persulcatus ticks, and we aimed to isolateB. miyamotoi from I. persulcatus from four regions of Greater Khingan, Inner Mongolia, China.
METHODS
METHODS
From May to June each year during the period 2016-2019, host-seeking adult I. persulcatus ticks were collected from vegetation. Genomic DNA was prepared from half of each tick body for PCR template, and the remaining half was used to cultivate B. miyamotoi in BSK-M medium. We employed quantitative real-time PCR (qPCR) to detect Borrelia DNA in the ticks and to calculate the prevalence of B. miyamotoi and infections with other borreliae. For characterization of the isolated B. miyamotoi, we performed draft genome sequencing and multilocus sequencing analysis (MLSA).
RESULTS
RESULTS
A total of 2656 adult I. persulcatus ticks were collected. The overall prevalence of relapsing fever (RF) borreliae in ticks was 5.0% (134/2656) and that of Lyme disease (LD) borreliae was 43.8% (1164/2656). Co-infection with RF and LD borreliae was observed in 63 ticks (2.4%). Ticks that were positive for RF borreliae by qPCR were subjected to glycerophosphodiester diester phosphodiesterase gene (glpQ) PCR amplification and sequencing, through which we identified the RF borrelia specimens as B. miyamotoi. Furthermore, the B. miyamotoi strain Hetao-1 was isolated from I. persulcatus, and a draft genome sequence was obtained from the isolate. Sequencing determined the strain Hetao-1 genome to be approximately 906.1 kbp in length (28.9% average GC content), and MLSA identified the strain as ST633, which has previously been reported in Japan and Mongolia.
CONCLUSION
CONCLUSIONS
We detected B. miyamotoi from I. persulcatus ticks collected in Inner Mongolia, and successfully isolated a B. miyamotoi strain. To our knowledge, this is the first study to culture a B. miyamotoi isolate from China. The data on the prevalence of B. miyamotoi and other borreliae in I. persulcatus ticks will be fundamental for future epidemiological studies of B. miyamotoi disease in Inner Mongolia.
Identifiants
pubmed: 34274015
doi: 10.1186/s13071-021-04809-z
pii: 10.1186/s13071-021-04809-z
pmc: PMC8285808
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
368Subventions
Organisme : National Natural Science Foundation of China
ID : 31660032
Organisme : National Natural Science Foundation of China
ID : 31660044
Organisme : Inner Mongolia Science and Technology Talent Project for Youth
ID : NJYT-18-A19
Organisme : Bayan Nur Doctoral Scientific Research Station
ID : BKZ2016
Organisme : Japan Agency for Medical Research and Development
ID : JP20wm0225016
Organisme : Japan Agency for Medical Research and Development
ID : JP20fk0108068
Organisme : Japan Agency for Medical Research and Development
ID : 21fk0108614
Informations de copyright
© 2021. The Author(s).
Références
J Mol Evol. 1980 Dec;16(2):111-20
pubmed: 7463489
Environ Microbiol Rep. 2011 Oct;3(5):632-7
pubmed: 23761345
Emerg Infect Dis. 2020 Sep;26(9):2201-2204
pubmed: 32818401
J Med Microbiol. 2007 Nov;56(Pt 11):1467-1473
pubmed: 17965346
Emerg Infect Dis. 2011 Oct;17(10):1816-23
pubmed: 22000350
Ticks Tick Borne Dis. 2016 Oct;7(6):1230-1235
pubmed: 27475875
N Engl J Med. 2013 Jan 17;368(3):240-5
pubmed: 23323900
N Engl J Med. 2013 Jan 17;368(3):291-3
pubmed: 23323920
Mol Biol Evol. 2013 Dec;30(12):2725-9
pubmed: 24132122
J Comput Biol. 2012 May;19(5):455-77
pubmed: 22506599
Emerg Infect Dis. 2018 May;24(5):928-931
pubmed: 29664385
Yale J Biol Med. 1984 Jul-Aug;57(4):521-5
pubmed: 6393604
Emerg Infect Dis. 2014 Aug;20(8):1391-3
pubmed: 25061761
PLoS One. 2014 Aug 11;9(8):e104532
pubmed: 25111141
Clin Microbiol Rev. 2006 Jan;19(1):165-256
pubmed: 16418529
PLoS One. 2013;8(3):e60843
pubmed: 23556006
Am J Trop Med Hyg. 2009 Dec;81(6):1120-31
pubmed: 19996447
Clin Microbiol Infect. 2015 Jul;21(7):631-9
pubmed: 25700888
J Med Entomol. 1990 Jul;27(4):646-50
pubmed: 2388239
BMC Genomics. 2020 Jan 6;21(1):16
pubmed: 31906865
Emerg Infect Dis. 2019 Oct;25(10):1965-1968
pubmed: 31538916
Emerg Infect Dis. 2018 Feb;24(2):236-241
pubmed: 29350133
PLoS One. 2012;7(12):e51914
pubmed: 23251652
Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8730-5
pubmed: 18574151
Emerg Infect Dis. 2016 Sep;22(9):1617-20
pubmed: 27533748
BMC Vet Res. 2011 Sep 20;7:56
pubmed: 21933420
Vector Borne Zoonotic Dis. 2011 Jul;11(7):877-82
pubmed: 21870355
Genome Res. 2010 Feb;20(2):265-72
pubmed: 20019144
Int J Syst Bacteriol. 1995 Oct;45(4):804-10
pubmed: 7547303
J Med Entomol. 2006 Jan;43(1):120-3
pubmed: 16506458
Ticks Tick Borne Dis. 2017 Oct;8(6):850-857
pubmed: 28768603
J Appl Microbiol. 2016 Feb;120(2):379-87
pubmed: 26606545
Ticks Tick Borne Dis. 2014 Oct;5(6):841-7
pubmed: 25108784
Genome Res. 2009 Jun;19(6):1117-23
pubmed: 19251739
Lancet. 2013 Aug 17;382(9892):658
pubmed: 23953389
Ticks Tick Borne Dis. 2018 Mar;9(3):465-470
pubmed: 29329785
Acta Trop. 2021 May;217:105857
pubmed: 33582142