Surface architecture of Neisseria meningitidis capsule and outer membrane as revealed by atomic force microscopy.
AFM
Capsule
Neisseria meningitidis
Outer membrane
Journal
Research in microbiology
ISSN: 1769-7123
Titre abrégé: Res Microbiol
Pays: France
ID NLM: 8907468
Informations de publication
Date de publication:
Historique:
received:
03
03
2021
revised:
03
07
2021
accepted:
06
07
2021
pubmed:
21
7
2021
medline:
29
12
2021
entrez:
20
7
2021
Statut:
ppublish
Résumé
An extensive morphological analysis of the Neisseria meningitidis cell envelope, including serogroup B capsule and outer membrane, based on atomic force microscopy (AFM) together with mechanical characterization by force spectroscopic measurements, has been carried out. Three meningococcal strains were used: the encapsulated serogroup B strain B1940, and the isogenic mutants B1940 siaD(+C) (lacking capsule), and B1940 cps (lacking both capsule and lipooligosaccharide outer core). AFM experiments with the encapsulated strain B1940 provided unprecedented images of the meningococcal capsule, which seems to be characterized by protrusions ("bumps") with the lateral dimensions of about 30 nm. Measurement of the Young's modulus provided quantitative assessment of the property of the capsule to confer resistance to mechanical stress. Moreover, Raman spectroscopy gave a fingerprint by which it was possible to identify the specific molecular species of the three strains analyzed, and to highlight major differences between them.
Identifiants
pubmed: 34284091
pii: S0923-2508(21)00079-6
doi: 10.1016/j.resmic.2021.103865
pii:
doi:
Substances chimiques
Polysaccharides, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
103865Informations de copyright
Copyright © 2021 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare no conflict of interest.