Upregulation of OGT by Caveolin-1 promotes hepatocellular carcinoma cell migration and invasion.
Carcinoma, Hepatocellular
/ genetics
Caveolin 1
/ genetics
Cell Line, Tumor
Cell Movement
/ genetics
Cell Proliferation
/ genetics
Core Binding Factor Alpha 1 Subunit
/ metabolism
Gene Expression
/ genetics
Gene Expression Regulation, Neoplastic
/ genetics
Glycosylation
Hep G2 Cells
Humans
Liver Neoplasms
/ genetics
MicroRNAs
/ genetics
N-Acetylglucosaminyltransferases
/ genetics
Neoplasm Invasiveness
/ genetics
Protein Processing, Post-Translational
Transcriptional Activation
CAV1
HCC
O-GlcNAc
OGT
Journal
Cell biology international
ISSN: 1095-8355
Titre abrégé: Cell Biol Int
Pays: England
ID NLM: 9307129
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
revised:
26
05
2021
received:
07
12
2020
accepted:
03
07
2021
pubmed:
22
7
2021
medline:
17
2
2022
entrez:
21
7
2021
Statut:
ppublish
Résumé
Caveolin-1 (CAV1), a major structural protein of caveolae, is reported to exert a positive regulatory effect on tumor growth and to play a crucial role in hepatocellular carcinoma (HCC) cell metastasis by regulating glycosyltransferase expression and cellular glycosylation. However, the role of CAV1 in modulating protein glycosylation and tumor metastasis remains to be further elucidated. In the present study, we showed that CAV1 promoted the expression of O-GlcNAc transferase (OGT), which catalyzed the addition of O-GlcNAc residues to a variety of nuclear and cytoplasmic proteins. In human HCC cell lines with different metastatic potentials, high levels of OGT and cellular O-GlcNAcylation were associated with CAV1 expression and cell metastasis. Overexpression of CAV1 increased the levels of OGT and O-GlcNAcylation, and cell migration was also increased. Furthermore, CAV1 was found to reduce the expression of Runt-related transcription factor 2 (RUNX2) in HCC cells. Subsequently, this effect resulted in the attenuation of the RUNX2-induced transcription of microRNA24 (miR24), a microRNA previously shown to suppress OGT mRNA expression by targeting its 3' untranslated regions (UTR). Finally, we demonstrated that CAV1 induced cellular O-GlcNAcylation and HCC cell invasion. This study provides evidence of CAV1-mediated increases in OGT expression and O-GlcNAcylation. These data provide insight into a novel mechanism underlying HCC metastasis and suggest a novel strategy for the treatment of HCC.
Substances chimiques
CAV1 protein, human
0
Caveolin 1
0
Core Binding Factor Alpha 1 Subunit
0
MIRN24 microRNA, human
0
MicroRNAs
0
RUNX2 protein, human
0
N-Acetylglucosaminyltransferases
EC 2.4.1.-
O-GlcNAc transferase
EC 2.4.1.-
OGT protein, human
EC 2.4.1.255
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2251-2263Subventions
Organisme : Fundamental Research Funds for the Central Universities
ID : DUT20YG116
Organisme : Fundamental Research Funds for the Central Universities
ID : DUT20YG130
Organisme : Natural Science Foundation of Liaoning Province
ID : 2019-MS-042
Organisme : National Science and Technology Major Project of China
ID : 2018ZX10302205
Organisme : Natural Science Foundation of China
ID : 31870793
Organisme : Natural Science Foundation of China
ID : 31971214
Informations de copyright
© 2021 International Federation for Cell Biology.
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