Anti-Apoptotic and Antioxidant Activities of the Mitochondrial Estrogen Receptor Beta in N2A Neuroblastoma Cells.
Animals
Antioxidants
/ metabolism
Apoptosis
/ drug effects
Cell Death
/ drug effects
Cell Line, Tumor
Estradiol
/ pharmacology
Estrogen Receptor beta
/ genetics
Estrogens
/ metabolism
Hydrogen Peroxide
/ metabolism
Mice
Mitochondria
/ metabolism
Neural Stem Cells
/ metabolism
Neuroblastoma
/ genetics
Neurons
/ metabolism
Neuroprotection
/ drug effects
Oxidative Stress
/ drug effects
Receptors, Estrogen
/ metabolism
apoptosis
estrogen receptor beta
mitochondria
neuroprotection
oxidative stress
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
16 Jul 2021
16 Jul 2021
Historique:
received:
07
06
2021
revised:
05
07
2021
accepted:
13
07
2021
entrez:
24
7
2021
pubmed:
25
7
2021
medline:
13
8
2021
Statut:
epublish
Résumé
Estrogens are steroid hormones that play a crucial role in the regulation of the reproductive and non-reproductive system physiology. Among non-reproductive systems, the nervous system is mainly affected by estrogens due to their antioxidant, anti-apoptotic, and anti-inflammatory activities, which are mediated by membranous and nuclear estrogen receptors, and also by non-estrogen receptor-associated estrogen actions. Neuronal viability and functionality are also associated with the maintenance of mitochondrial functions. Recently, the localization of estrogen receptors, especially estrogen receptor beta, in the mitochondria of many types of neuronal cells is documented, indicating the direct involvement of the mitochondrial estrogen receptor beta (mtERβ) in the maintenance of neuronal physiology. In this study, cell lines of N2A cells stably overexpressing a mitochondrial-targeted estrogen receptor beta were generated and further analyzed to study the direct involvement of mtERβ in estrogen neuroprotective antioxidant and anti-apoptotic actions. Results from this study revealed that the presence of estrogen receptor beta in mitochondria render N2A cells more resistant to staurosporine- and H
Identifiants
pubmed: 34299239
pii: ijms22147620
doi: 10.3390/ijms22147620
pmc: PMC8306648
pii:
doi:
Substances chimiques
Antioxidants
0
Estrogen Receptor beta
0
Estrogens
0
Receptors, Estrogen
0
Estradiol
4TI98Z838E
Hydrogen Peroxide
BBX060AN9V
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Regional Development Fund
ID : MIS 5002636
Organisme : Hellenic Foundation for Research and Innovation
ID : 6246
Organisme : University of Thessaly
ID : 3439
Organisme : University of Thessaly
ID : 3818
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