Impact of Peels Extracts from an Italian Ancient Tomato Variety Grown under Drought Stress Conditions on Vascular Related Dysfunction.
drought stress
human vascular endothelial cells
oxidative stress
polyphenols
tomato by-products
tomato peel extract
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
15 Jul 2021
15 Jul 2021
Historique:
received:
24
06
2021
revised:
11
07
2021
accepted:
13
07
2021
entrez:
24
7
2021
pubmed:
25
7
2021
medline:
28
9
2021
Statut:
epublish
Résumé
Tomato by-products contain a great variety of biologically active substances and represent a significant source of natural antioxidant supplements of the human diet. The aim of the work was to compare the antioxidant properties of a by-product from an ancient Tuscan tomato variety, Rosso di Pitigliano (RED), obtained by growing plants in normal conditions (-Ctr) or in drought stress conditions (-Ds) for their beneficial effects on vascular related dysfunction. The antioxidant activity and total polyphenol content (TPC) were measured. The identification of bioactive compounds of tomato peel was performed by HPLC. HUVEC were pre-treated with different TPC of RED-Ctr or RED-Ds, then stressed with H RED-Ds tomato peel extract possessed higher TPC than compared to RED-Ctr (361.32 ± 7.204 mg vs. 152.46 ± 1.568 mg GAE/100 g fresh weight). All extracts were non-cytotoxic. Two hour pre-treatment with 5 µg GAE/mL from RED-Ctr or RED-Ds showed protection from H RED-Ds tomato peel extract represents a good source of bioactive molecules, which protects HUVECs from oxidative stress at low concentration.
Sections du résumé
BACKGROUND
BACKGROUND
Tomato by-products contain a great variety of biologically active substances and represent a significant source of natural antioxidant supplements of the human diet. The aim of the work was to compare the antioxidant properties of a by-product from an ancient Tuscan tomato variety, Rosso di Pitigliano (RED), obtained by growing plants in normal conditions (-Ctr) or in drought stress conditions (-Ds) for their beneficial effects on vascular related dysfunction.
METHODS
METHODS
The antioxidant activity and total polyphenol content (TPC) were measured. The identification of bioactive compounds of tomato peel was performed by HPLC. HUVEC were pre-treated with different TPC of RED-Ctr or RED-Ds, then stressed with H
RESULTS
RESULTS
RED-Ds tomato peel extract possessed higher TPC than compared to RED-Ctr (361.32 ± 7.204 mg vs. 152.46 ± 1.568 mg GAE/100 g fresh weight). All extracts were non-cytotoxic. Two hour pre-treatment with 5 µg GAE/mL from RED-Ctr or RED-Ds showed protection from H
CONCLUSIONS
CONCLUSIONS
RED-Ds tomato peel extract represents a good source of bioactive molecules, which protects HUVECs from oxidative stress at low concentration.
Identifiants
pubmed: 34299564
pii: molecules26144289
doi: 10.3390/molecules26144289
pmc: PMC8307906
pii:
doi:
Substances chimiques
Antioxidants
0
Plant Extracts
0
Polyphenols
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
J Sci Food Agric. 2018 Mar;98(4):1271-1279
pubmed: 28799642
J Appl Toxicol. 2017 Dec;37(12):1359-1369
pubmed: 28383141
Molecules. 2010 Feb 23;15(2):959-87
pubmed: 20335956
J Sep Sci. 2008 Feb;31(2):262-7
pubmed: 18172921
Free Radic Biol Med. 2004 Apr 15;36(8):1058-67
pubmed: 15059646
Plant Physiol Biochem. 2016 Aug;105:79-89
pubmed: 27085599
Mol Nutr Food Res. 2007 May;51(5):609-17
pubmed: 17427261
Br J Pharmacol. 2017 Jun;174(11):1290-1324
pubmed: 27638711
Int J Pharm. 2005 Mar 3;291(1-2):183-8
pubmed: 15707745
Crit Rev Food Sci Nutr. 2015;55(7):919-29
pubmed: 24915375
Int J Prev Med. 2014 Dec;5(12):1487-99
pubmed: 25709784
J Agric Food Chem. 2007 Aug 8;55(16):6475-81
pubmed: 17629300
Plants (Basel). 2019 Sep 07;8(9):
pubmed: 31500309
J Agric Food Chem. 2007 Oct 17;55(21):8604-15
pubmed: 17927150
Anal Biochem. 1996 Jul 15;239(1):70-6
pubmed: 8660627
J Med Food. 2013 Apr;16(4):351-6
pubmed: 23472745
Curr Opin Biotechnol. 2010 Apr;21(2):197-203
pubmed: 20363612
Plant Physiol Biochem. 2009 Jul;47(7):570-7
pubmed: 19318268
J Sci Food Agric. 2014 Mar 30;94(5):993-9
pubmed: 24037905
Am J Clin Nutr. 2012 May;95(5):1013-22
pubmed: 22492370
Nutrients. 2015 Nov 19;7(11):9640-9
pubmed: 26610560
Front Plant Sci. 2014 Mar 13;5:86
pubmed: 24659993
Eur J Pharm Sci. 2018 Jan 1;111:425-431
pubmed: 29055735
Nutrients. 2020 Aug 28;12(9):
pubmed: 32872374
Front Pharmacol. 2018 May 23;9:521
pubmed: 29875663
Crit Rev Food Sci Nutr. 2014;54(8):1032-49
pubmed: 24499120
Front Plant Sci. 2016 Jul 14;7:1029
pubmed: 27471513
Foods. 2017 Jul 25;6(8):
pubmed: 28757563
Oxid Med Cell Longev. 2013;2013:516051
pubmed: 23577223
J Agric Food Chem. 2013 Oct 9;61(40):9534-50
pubmed: 24079774
Eur J Pharm Biopharm. 2010 Jun;75(2):194-9
pubmed: 20171277
Antioxidants (Basel). 2020 Feb 03;9(2):
pubmed: 32028655
Int J Food Sci Nutr. 2009 Dec;60(8):694-708
pubmed: 19919517
J Sep Sci. 2007 Mar;30(4):452-61
pubmed: 17444214
J Clin Invest. 1973 Nov;52(11):2745-56
pubmed: 4355998
Oxid Med Cell Longev. 2019 Jun 23;2019:7092151
pubmed: 31341533
Nutrients. 2018 Nov 01;10(11):
pubmed: 30388730
J Zhejiang Univ Sci B. 2010 May;11(5):357-65
pubmed: 20443214
Int J Mol Sci. 2019 Nov 25;20(23):
pubmed: 31775339
Ann Nutr Metab. 2003;47(2):64-9
pubmed: 12652057
Rapid Commun Mass Spectrom. 2010 Oct 30;24(20):2986-92
pubmed: 20872631
Eur J Pharm Biopharm. 2012 Jan;80(1):176-84
pubmed: 21971368
Plant Foods Hum Nutr. 2018 Dec;73(4):268-277
pubmed: 30264237
J Agric Food Chem. 2011 Sep 14;59(17):9673-82
pubmed: 21830786
J Sci Food Agric. 2013 Apr;93(6):1449-57
pubmed: 23070982