Arrest of WNT/β-catenin signaling enables the transition from pluripotent to differentiated germ cells in mouse ovaries.
POU5F1/OCT4
WNT/β-catenin
differentiation
germ cells
ovary
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
27 07 2021
27 07 2021
Historique:
entrez:
24
7
2021
pubmed:
25
7
2021
medline:
15
12
2021
Statut:
ppublish
Résumé
Germ cells form the basis for sexual reproduction by producing gametes. In ovaries, primordial germ cells exit the cell cycle and the pluripotency-associated state, differentiate into oogonia, and initiate meiosis. Despite the importance of germ cell differentiation for sexual reproduction, signaling pathways regulating their fate remain largely unknown. Here, we show in mouse embryonic ovaries that germ cell-intrinsic β-catenin activity maintains pluripotency and that its repression is essential to allow differentiation and meiosis entry in a timely manner. Accordingly, in β-catenin loss-of-function and gain-of-function mouse models, the germ cells precociously enter meiosis or remain in the pluripotent state, respectively. We further show that interaction of β-catenin and the pluripotent-associated factor POU5F1 in the nucleus is associated with germ cell pluripotency. The exit of this complex from the nucleus correlates with germ cell differentiation, a process promoted by the up-regulation of
Identifiants
pubmed: 34301885
pii: 2023376118
doi: 10.1073/pnas.2023376118
pmc: PMC8325354
pii:
doi:
Substances chimiques
CTNNB1 protein, mouse
0
Octamer Transcription Factor-3
0
Pou5f1 protein, mouse
0
Wnt Proteins
0
beta Catenin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Medical Research Council
ID : MC_U142684167
Pays : United Kingdom
Informations de copyright
Copyright © 2021 the Author(s). Published by PNAS.
Déclaration de conflit d'intérêts
The authors declare no competing interest.
Références
Nature. 2012 Aug 30;488(7413):665-9
pubmed: 22895187
Genes Dev. 2008 Mar 15;22(6):746-55
pubmed: 18347094
Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4453-8
pubmed: 15070739
Curr Drug Targets. 2006 Nov;7(11):1377-88
pubmed: 17100578
Nat Rev Cancer. 2019 Sep;19(9):522-537
pubmed: 31413324
Development. 2001 Apr;128(8):1253-64
pubmed: 11262227
Nature. 2000 Jul 6;406(6791):86-90
pubmed: 10894547
EMBO Rep. 2004 Nov;5(11):1078-83
pubmed: 15486564
Exp Cell Res. 1996 Feb 1;222(2):291-7
pubmed: 8598216
Cell. 2006 Nov 3;127(3):469-80
pubmed: 17081971
Dev Biol. 2003 Oct 15;262(2):303-12
pubmed: 14550793
Science. 2020 Mar 6;367(6482):
pubmed: 32054698
Stem Cell Reports. 2017 Mar 14;8(3):561-575
pubmed: 28196692
Nature. 2012 Apr 29;485(7397):195-200
pubmed: 22575959
Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2474-9
pubmed: 16461896
Science. 2000 Mar 3;287(5458):1606-9
pubmed: 10733430
J Biol Chem. 2002 Sep 6;277(36):33398-410
pubmed: 12052822
J Pathol. 2004 Oct;204(2):167-74
pubmed: 15378486
Science. 2006 Apr 28;312(5773):596-600
pubmed: 16574820
Nature. 1999 Feb 4;397(6718):405-9
pubmed: 9989404
Nat Genet. 2006 Dec;38(12):1430-4
pubmed: 17115059
Proc Natl Acad Sci U S A. 2013 Oct 8;110(41):16492-7
pubmed: 24067652
Cell Stem Cell. 2011 Feb 4;8(2):214-27
pubmed: 21295277
Nature. 1990 Jun 21;345(6277):686-92
pubmed: 1972777
Dev Biol. 1997 Jul 1;187(1):107-13
pubmed: 9224678
FASEB J. 2021 Apr;35(4):e21452
pubmed: 33749946
EMBO J. 2017 Nov 2;36(21):3100-3119
pubmed: 28928204
Dev Biol. 2003 Oct 1;262(1):1-15
pubmed: 14512014
FEBS Lett. 2007 Nov 13;581(27):5247-54
pubmed: 17950287
Development. 2006 Sep;133(17):3411-8
pubmed: 16887828
Mol Cell. 2008 Mar 28;29(6):755-66
pubmed: 18374649
Trends Biochem Sci. 2010 Mar;35(3):161-8
pubmed: 19884009
Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9485-90
pubmed: 10449719
PLoS Biol. 2016 Sep 08;14(9):e1002553
pubmed: 27606421
Genes Dev. 2008 Mar 1;22(5):575-80
pubmed: 18283123
Genes Dev. 2012 May 1;26(9):920-32
pubmed: 22499591
Genesis. 2007 Sep;45(9):593-605
pubmed: 17868096
J Exp Zool. 1983 Nov;228(2):173-93
pubmed: 6663256
Trends Cell Biol. 2012 Mar;22(3):159-68
pubmed: 22196214
PLoS Biol. 2020 Dec 22;18(12):e3001025
pubmed: 33351795
Cold Spring Harb Perspect Biol. 2012 Nov 01;4(11):
pubmed: 23125014
Cytogenet Genome Res. 2003;101(3-4):261-5
pubmed: 14684992
Dev Biol. 2008 May 15;317(2):576-84
pubmed: 18395706
Biol Reprod. 2009 Nov;81(5):996-1001
pubmed: 19587328
Dev Growth Differ. 2018 Dec;60(9):542-552
pubmed: 30443901
Proc Natl Acad Sci U S A. 2011 May 3;108(18):7443-8
pubmed: 21504946
Sci Adv. 2020 May 22;6(21):eaaz1261
pubmed: 32494737
Nature. 2008 Jul 3;454(7200):109-13
pubmed: 18568026
Proc Natl Acad Sci U S A. 2020 Aug 18;117(33):20015-20026
pubmed: 32759216
PLoS Genet. 2015 Mar 04;11(3):e1005019
pubmed: 25739037
Cell Stem Cell. 2012 Sep 7;11(3):387-400
pubmed: 22863533
Proc Natl Acad Sci U S A. 2016 Mar 15;113(11):E1489-97
pubmed: 26929341
Development. 2002 Mar;129(5):1155-64
pubmed: 11874911
Mol Reprod Dev. 2004 Aug;68(4):422-8
pubmed: 15236325
Curr Top Dev Biol. 2014;107:373-404
pubmed: 24439813
Dev Biol. 2006 Dec 15;300(2):545-53
pubmed: 17055474
Sci Adv. 2020 May 22;6(21):
pubmed: 32917583
PLoS One. 2011;6(10):e25641
pubmed: 21991325
Proc Natl Acad Sci U S A. 2018 May 22;115(21):5474-5479
pubmed: 29735715
Mol Cell Biol. 2002 Feb;22(4):1184-93
pubmed: 11809809
Nature. 2018 Mar 15;555(7696):392-396
pubmed: 29513657
EMBO Rep. 2013 May;14(5):422-33
pubmed: 23598517
Nucleic Acids Res. 2018 Apr 6;46(6):2850-2867
pubmed: 29394407
Dev Biol. 2013 Nov 15;383(2):295-306
pubmed: 24036309
Biol Reprod. 2012 Feb 14;86(2):37
pubmed: 21976597
Mol Cell. 2016 Aug 18;63(4):647-661
pubmed: 27499297
Cell Stem Cell. 2011 Oct 4;9(4):317-29
pubmed: 21982232
Dev Cell. 2020 Feb 24;52(4):429-445.e10
pubmed: 32032549
PLoS Genet. 2019 Sep 19;15(9):e1008333
pubmed: 31537017
Hum Mol Genet. 2008 May 1;17(9):1264-77
pubmed: 18250098
Development. 1990 May;109(1):37-40
pubmed: 2209469
Development. 2012 Jan;139(1):15-31
pubmed: 22147951
J Biol Chem. 2002 May 17;277(20):17901-5
pubmed: 11834740
Stem Cells. 2007 Feb;25(2):500-10
pubmed: 17068183
Hum Mol Genet. 2010 Apr 15;19(8):1539-50
pubmed: 20106871
Dev Biol. 2017 Jun 1;426(1):17-27
pubmed: 28456466
Biol Reprod. 2015 Aug;93(2):35
pubmed: 26108792
Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):16061-6
pubmed: 24043772
Nature. 2013 Mar 14;495(7440):236-40
pubmed: 23486062