CD97 stabilises the immunological synapse between dendritic cells and T cells and is targeted for degradation by the Salmonella effector SteD.
Animals
Antigen Presentation
/ immunology
Bacterial Proteins
/ metabolism
Dendritic Cells
/ immunology
Immunological Synapses
/ immunology
Lymphocyte Activation
/ immunology
Mice
Mice, Inbred C57BL
Receptors, G-Protein-Coupled
/ immunology
Salmonella Infections
/ metabolism
Salmonella enterica
T-Lymphocytes
/ immunology
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
received:
22
04
2021
accepted:
29
06
2021
revised:
06
08
2021
pubmed:
28
7
2021
medline:
23
2
2023
entrez:
27
7
2021
Statut:
epublish
Résumé
The Salmonella enterica effector SteD depletes mature MHC class II (mMHCII) molecules from the surface of infected antigen-presenting cells through ubiquitination of the cytoplasmic tail of the mMHCII β chain. This requires the Nedd4 family HECT E3 ubiquitin ligase Wwp2 and a tumor-suppressing transmembrane protein adaptor Tmem127. Here, through a proteomic screen of dendritic cells, we found that SteD targets the plasma membrane protein CD97 for degradation by a similar mechanism. SteD enhanced ubiquitination of CD97 on K555 and mutation of this residue eliminated the effect of SteD on CD97 surface levels. We showed that CD97 localises to and stabilises the immunological synapse between dendritic cells and T cells. Removal of CD97 by SteD inhibited dendritic cell-T cell interactions and reduced T cell activation, independently of its effect on MHCII. Therefore, SteD suppresses T cell immunity by two distinct processes.
Identifiants
pubmed: 34314469
doi: 10.1371/journal.ppat.1009771
pii: PPATHOGENS-D-21-00867
pmc: PMC8345877
doi:
Substances chimiques
Adgre5 protein, mouse
0
Bacterial Proteins
0
Receptors, G-Protein-Coupled
0
SteD protein, Salmonella typhimurium
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1009771Subventions
Organisme : Wellcome Trust
ID : 210688/Z/18/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/V011561/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 209411/Z/17/Z
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 084957/Z/08/Z
Pays : United Kingdom
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
J Cell Biol. 2009 May 4;185(3):493-502
pubmed: 19398763
Handb Exp Pharmacol. 2016;234:329-350
pubmed: 27832495
Int Immunol. 2000 Apr;12(4):439-48
pubmed: 10744645
Infect Immun. 2019 Oct 18;87(11):
pubmed: 31427450
Infect Immun. 2007 Mar;75(3):1144-53
pubmed: 17158902
Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):14052-7
pubmed: 19666567
EMBO J. 2006 Apr 19;25(8):1635-45
pubmed: 16601694
Cell Rep. 2018 Jul 31;24(5):1151-1162
pubmed: 30067972
J Exp Med. 2006 Jun 12;203(6):1407-12
pubmed: 16717117
Annu Rev Immunol. 2013;31:563-604
pubmed: 23516985
Nat Genet. 2014 Dec;46(12):1333-6
pubmed: 25383971
J Biol Chem. 2007 Jul 27;282(30):22023-32
pubmed: 17449467
Proc Natl Acad Sci U S A. 2013 Sep 24;110(39):15722-7
pubmed: 24019463
J Biol Chem. 2001 Jun 29;276(26):24160-9
pubmed: 11297558
Nat Rev Immunol. 2019 Feb;19(2):89-103
pubmed: 30464294
Cell Host Microbe. 2016 Nov 9;20(5):584-595
pubmed: 27832589
Cell Host Microbe. 2020 Jul 8;28(1):54-68.e7
pubmed: 32526160
Immunol Lett. 2019 Nov;215:35-39
pubmed: 30771380
Cell Microbiol. 2020 May;22(5):e13166
pubmed: 31957253
Front Immunol. 2012 Nov 07;3:331
pubmed: 23162549
Nat Immunol. 2000 Jul;1(1):23-9
pubmed: 10881170
Front Immunol. 2019 Jun 20;10:1412
pubmed: 31281320
Nat Methods. 2013 Aug;10(8):730-6
pubmed: 23921808
Infect Immun. 2006 Aug;74(8):4922-6
pubmed: 16861683
J Leukoc Biol. 2005 Jan;77(1):112-9
pubmed: 15498814
Cell Microbiol. 2001 Sep;3(9):587-97
pubmed: 11553011
J Immunol. 2008 Nov 1;181(9):6574-83
pubmed: 18941248
Cell Host Microbe. 2015 Oct 14;18(4):409-23
pubmed: 26439863
Infect Immun. 2014 Dec;82(12):4997-5004
pubmed: 25225248
Future Microbiol. 2015;10(1):101-10
pubmed: 25598340
Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6020-4
pubmed: 1378619
Infect Immun. 2002 Jan;70(1):199-203
pubmed: 11748183
Nat Methods. 2014 Aug;11(8):783-784
pubmed: 25075903
Adv Exp Med Biol. 2010;706:128-37
pubmed: 21618832
Blood. 2005 Apr 1;105(7):2836-44
pubmed: 15576472
J Cell Biol. 2015 Feb 16;208(4):457-73
pubmed: 25666808
Front Immunol. 2018 Jan 18;9:25
pubmed: 29403502
Cell Signal. 2003 Aug;15(8):793-802
pubmed: 12781872
Cell Host Microbe. 2017 Aug 9;22(2):217-231
pubmed: 28799907
Cancer Immunol Res. 2014 Nov;2(11):1023-33
pubmed: 25367977
Subcell Biochem. 2006;40:383-6
pubmed: 17623925
J Biol Chem. 2004 Jul 23;279(30):31823-32
pubmed: 15150276
J Immunol. 2006 Jul 15;177(2):1070-7
pubmed: 16818763