Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West Africa.
Anopheles gambiae
Bendiocarb
Insecticide resistance
Intensity
Pyrethroids
Journal
Malaria journal
ISSN: 1475-2875
Titre abrégé: Malar J
Pays: England
ID NLM: 101139802
Informations de publication
Date de publication:
27 Jul 2021
27 Jul 2021
Historique:
received:
13
01
2021
accepted:
16
07
2021
entrez:
28
7
2021
pubmed:
29
7
2021
medline:
6
11
2021
Statut:
epublish
Résumé
The selection and the spread of insecticide resistance in malaria vectors to the main classes of insecticides used in vector control tools are a major and ongoing challenge to malaria vector control programmes. This study aimed to determine the intensity of vector resistance to insecticides in three regions of Benin with different agro-ecological characteristics. Larvae of Anopheles gambiae sensu lato (s.l.) were collected from September to November 2017 in different larval sites in three northern Benin communes: Parakou, Kandi and Malanville. Two to five-day-old, non-blood-fed, female mosquitoes were exposed to papers impregnated with deltamethrin, permethrin and bendiocarb at dosages of 1 × the diagnostic dose, 5 × and 10 × to determine the intensity of resistance in these vectors. Molecular frequencies of the kdr L1014F and ace-1R G119S insecticide resistance mutations and levels of detoxification enzymes were determined for mosquitoes sampled at each study site. Resistance to pyrethroids (permethrin and deltamethrin) was recorded in all three communes with mortality rates below 60% using the diagnostic dose (1x). The results obtained after exposure of An. gambiae to permethrin 10 × were 99% in Kandi, 98% in Malanville and 99% in Parakou. With deltamethrin 10x, mortality rates were 100% in Kandi, 96% in Malanville and 73% in Parakou. For the diagnostic dose of bendiocarb, suspected resistance was recorded in the communes of Malanville (97%) and Kandi (94%) while sensitivity was observed in Parakou (98%).Using the 10 × dose, mortality was 98% in Kandi, 100% in Malanville and 99% in Parakou. The frequencies of the kdr L1014F allele varied between 59 and 83% depending on the sites and species of the An. gambiae complex, while the frequency of the ace-1R G119S gene varied between 0 and 5%. Biochemical tests showed high levels of oxidase and esterase activity compared to the susceptible colony strain of An. gambiae sensu stricto (Kisumu strain). Anopheles gambiae showed a generalized loss of susceptibility to permethrin and deltamethrin but also showed moderate to high intensity of resistance in different regions of Benin. This high intensity of resistance is a potential threat to the effectiveness of vector control.
Sections du résumé
BACKGROUND
BACKGROUND
The selection and the spread of insecticide resistance in malaria vectors to the main classes of insecticides used in vector control tools are a major and ongoing challenge to malaria vector control programmes. This study aimed to determine the intensity of vector resistance to insecticides in three regions of Benin with different agro-ecological characteristics.
METHODS
METHODS
Larvae of Anopheles gambiae sensu lato (s.l.) were collected from September to November 2017 in different larval sites in three northern Benin communes: Parakou, Kandi and Malanville. Two to five-day-old, non-blood-fed, female mosquitoes were exposed to papers impregnated with deltamethrin, permethrin and bendiocarb at dosages of 1 × the diagnostic dose, 5 × and 10 × to determine the intensity of resistance in these vectors. Molecular frequencies of the kdr L1014F and ace-1R G119S insecticide resistance mutations and levels of detoxification enzymes were determined for mosquitoes sampled at each study site.
RESULTS
RESULTS
Resistance to pyrethroids (permethrin and deltamethrin) was recorded in all three communes with mortality rates below 60% using the diagnostic dose (1x). The results obtained after exposure of An. gambiae to permethrin 10 × were 99% in Kandi, 98% in Malanville and 99% in Parakou. With deltamethrin 10x, mortality rates were 100% in Kandi, 96% in Malanville and 73% in Parakou. For the diagnostic dose of bendiocarb, suspected resistance was recorded in the communes of Malanville (97%) and Kandi (94%) while sensitivity was observed in Parakou (98%).Using the 10 × dose, mortality was 98% in Kandi, 100% in Malanville and 99% in Parakou. The frequencies of the kdr L1014F allele varied between 59 and 83% depending on the sites and species of the An. gambiae complex, while the frequency of the ace-1R G119S gene varied between 0 and 5%. Biochemical tests showed high levels of oxidase and esterase activity compared to the susceptible colony strain of An. gambiae sensu stricto (Kisumu strain).
CONCLUSION
CONCLUSIONS
Anopheles gambiae showed a generalized loss of susceptibility to permethrin and deltamethrin but also showed moderate to high intensity of resistance in different regions of Benin. This high intensity of resistance is a potential threat to the effectiveness of vector control.
Identifiants
pubmed: 34315480
doi: 10.1186/s12936-021-03856-2
pii: 10.1186/s12936-021-03856-2
pmc: PMC8314583
doi:
Substances chimiques
Insecticides
0
Nitriles
0
Phenylcarbamates
0
Pyrethrins
0
decamethrin
2JTS8R821G
Permethrin
509F88P9SZ
bendiocarb
QFH0ZU0A5U
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
328Informations de copyright
© 2021. The Author(s).
Références
Parasit Vectors. 2013 Jun 26;6:192
pubmed: 23803527
Arch Insect Biochem Physiol. 1993;22(1-2):263-76
pubmed: 8431600
Parasit Vectors. 2015 Apr 12;8:223
pubmed: 25886599
Parasit Vectors. 2017 Nov 6;10(1):548
pubmed: 29110724
Malar J. 2010 Mar 24;9:83
pubmed: 20334637
Trop Med Int Health. 2008 Apr;13(4):476-86
pubmed: 18248566
Cochrane Database Syst Rev. 2018 Nov 29;11:CD012776
pubmed: 30488945
Malar J. 2006 Mar 24;5:22
pubmed: 16563153
Trop Med Int Health. 2008 Dec;13(12):1479-87
pubmed: 18983277
Malar J. 2018 Oct 29;17(1):392
pubmed: 30373576
Am J Trop Med Hyg. 2002 Dec;67(6):617-22
pubmed: 12518852
Am J Trop Med Hyg. 2010 Aug;83(2):266-70
pubmed: 20682865
Malar J. 2011 Sep 12;10:261
pubmed: 21910856
Acta Trop. 2010 May;114(2):116-22
pubmed: 20138819
Acta Trop. 2007 Mar;101(3):207-16
pubmed: 17359927
Med Vet Entomol. 2013 Jun;27(2):156-64
pubmed: 22861380
Malar J. 2008 Apr 29;7:74
pubmed: 18445265
Insect Mol Biol. 1998 May;7(2):179-84
pubmed: 9535162
Insect Biochem Mol Biol. 2004 Jul;34(7):653-65
pubmed: 15242706
Malar J. 2009 May 14;8:103
pubmed: 19442297
Parasit Vectors. 2020 Mar 24;13(1):152
pubmed: 32209131
Parasit Vectors. 2019 Nov 15;12(1):544
pubmed: 31730481
Parasitol Today. 1988 Jul;4(7):S8-12
pubmed: 15463082
Parasit Vectors. 2013 Sep 21;6:274
pubmed: 24330550
Parasit Vectors. 2011 Apr 13;4:60
pubmed: 21489266
Emerg Infect Dis. 2007 Feb;13(2):199-206
pubmed: 17479880
Insect Mol Biol. 2004 Feb;13(1):1-7
pubmed: 14728661
PLoS Genet. 2021 Jan 21;17(1):e1009253
pubmed: 33476334