nsP4 Is a Major Determinant of Alphavirus Replicase Activity and Template Selectivity.
Alphavirus
/ genetics
Alphavirus Infections
/ genetics
Animals
Base Sequence
Cell Line
DNA-Directed RNA Polymerases
/ metabolism
Humans
Polyproteins
/ metabolism
RNA, Viral
/ metabolism
RNA-Dependent RNA Polymerase
/ genetics
Viral Nonstructural Proteins
/ genetics
Viral Replicase Complex Proteins
/ genetics
Virus Replication
/ genetics
RNA polymerases
RNA replication
alphavirus
genetic recombination
replicase
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
27 09 2021
27 09 2021
Historique:
pubmed:
29
7
2021
medline:
30
12
2021
entrez:
28
7
2021
Statut:
ppublish
Résumé
Alphaviruses have positive-strand RNA genomes containing two open reading frames (ORFs). The first ORF encodes the nonstructural (ns) polyproteins P123 and P1234 that act as precursors for the subunits of the viral RNA replicase (nsP1 to nsP4). Processing of P1234 leads to the formation of a negative-strand replicase consisting of nsP4 (RNA polymerase) and P123 components. Subsequent processing of P123 results in a positive-strand replicase. The second ORF encoding the structural proteins is expressed via the synthesis of a subgenomic RNA. Alphavirus replicase is capable of using template RNAs that contain essential
Identifiants
pubmed: 34319783
doi: 10.1128/JVI.00355-21
pmc: PMC8475546
doi:
Substances chimiques
Polyproteins
0
RNA, Viral
0
Viral Nonstructural Proteins
0
Viral Replicase Complex Proteins
0
RNA-Dependent RNA Polymerase
EC 2.7.7.48
DNA-Directed RNA Polymerases
EC 2.7.7.6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0035521Subventions
Organisme : UKRI | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BBS/E/I/00007033 BBS/E/I/00007038 and BBS/E/I/00007039
Organisme : Estonian Research Council
ID : PRG1154
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007039
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust (Wellcome)
ID : 200171/Z/15/Z
Organisme : EC | European Regional Development Fund (ERDF)
ID : 2014-2020.4.01.15-013
Organisme : Medical Research Council
ID : MC_PC_16028
Pays : United Kingdom
Organisme : Research Foundation Flanders
ID : KAN1526318N
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007038
Pays : United Kingdom
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