Optimized methods for detecting Salmonella Typhi in the environment using validated field sampling, culture and confirmatory molecular approaches.
Identification
Malawi
Moore swabs
PCR (polymerase chain reaction)
bile broth
biofilm
biofilms
mCASE
river water
salmonella
selenite broth
typhoid
water
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Feb 2022
Feb 2022
Historique:
revised:
07
07
2021
received:
05
05
2021
accepted:
08
07
2021
pubmed:
30
7
2021
medline:
22
1
2022
entrez:
29
7
2021
Statut:
ppublish
Résumé
This study evaluated detection methods for Salmonella Typhi (S. Typhi) in the environment, to establish a novel pathway from field sampling to isolation of viable organisms and molecular confirmation from complex environmental samples, thus enabling environmental surveillance of typhoid. Multiple media were assessed using clinical isolates from the Public Health England's (PHE) Culture collection. The culture pathway selected consisted of a primary 2% bile broth and secondary Selenite F broth, followed by modified Chromogenic Agar for Salmonella Esterase (mCASE). A qPCR assay was adapted from a validated S. Typhi PCR panel for confirmation of isolates, with comparison to biochemical and serological tests showing good specificity. Sampling locations in Blantyre, Malawi were used to compare sampling methods. Viable S. Typhi were isolated from a mixture of trap and grab river water samples on six occasions. Culture of viable S. Typhi from environmental samples was possible using effective capture and culture techniques. Whilst several studies have attempted to detect S. Typhi from the environment, this is the first successful attempt to isolate the organism from river water since the 1980s. Supplementing clinical data with environmental screening offers the potential for enhanced surveillance, which might inform interventions and assess vaccination programmes.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1503-1517Subventions
Organisme : Bill and Melinda Gates Foundation
ID : OPP1155752
Organisme : Malawi-Liverpool-Wellcome Trust
Organisme : Wellcome Trust
Pays : United Kingdom
Informations de copyright
© 2021 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.
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