Endomembrane targeting of human OAS1 p46 augments antiviral activity.


Journal

eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614

Informations de publication

Date de publication:
03 08 2021
Historique:
received: 08 06 2021
accepted: 11 06 2021
pubmed: 4 8 2021
medline: 18 8 2021
entrez: 3 8 2021
Statut: epublish

Résumé

Many host RNA sensors are positioned in the cytosol to detect viral RNA during infection. However, most positive-strand RNA viruses replicate within a modified organelle co-opted from intracellular membranes of the endomembrane system, which shields viral products from cellular innate immune sensors. Targeting innate RNA sensors to the endomembrane system may enhance their ability to sense RNA generated by viruses that use these compartments for replication. Here, we reveal that an isoform of oligoadenylate synthetase 1, OAS1 p46, is prenylated and targeted to the endomembrane system. Membrane localization of OAS1 p46 confers enhanced access to viral replication sites and results in increased antiviral activity against a subset of RNA viruses including flaviviruses, picornaviruses, and SARS-CoV-2. Finally, our human genetic analysis shows that the

Identifiants

pubmed: 34342578
doi: 10.7554/eLife.71047
pii: 71047
pmc: PMC8357416
doi:
pii:

Substances chimiques

OAS1 protein, human EC 2.7.7.-
2',5'-Oligoadenylate Synthetase EC 2.7.7.84

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI108765
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI150214
Pays : United States
Organisme : NIH HHS
ID : AI127463
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI106677
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL007312
Pays : United States
Organisme : NIH HHS
ID : AI145974
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI135437
Pays : United States
Organisme : NIH HHS
ID : AI104002
Pays : United States
Organisme : NIH HHS
ID : GM007270
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007270
Pays : United States
Organisme : NIH HHS
ID : AI118916
Pays : United States
Organisme : NIH HHS
ID : AI106677
Pays : United States
Organisme : NIAID NIH HHS
ID : F31 AI140530
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI145296
Pays : United States
Organisme : NIH HHS
ID : AI145974,AI108765,AI135437
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI084914
Pays : United States
Organisme : NIH HHS
ID : P51 OD010425
Pays : United States
Organisme : NIH HHS
ID : AI145296
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI118916
Pays : United States
Organisme : NIH HHS
ID : AI108765
Pays : United States
Organisme : NIH HHS
ID : AI106677,GM007270,AI140530
Pays : United States
Organisme : NIH HHS
ID : T32 HL007312
Pays : United States
Organisme : NIAID NIH HHS
ID : R56 AI145974
Pays : United States
Organisme : Medical Research Council
ID : MC_PC_20004
Pays : United Kingdom
Organisme : NIH HHS
ID : AI135437
Pays : United States
Organisme : NIH HHS
ID : AI100625
Pays : United States
Organisme : NIH HHS
ID : S10 OD026741
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI104002
Pays : United States
Organisme : NIH HHS
ID : AI104002,AI118916,AI145296,AI127463,AI100625
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI127463
Pays : United States
Organisme : NIH HHS
ID : AI140530
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI100625
Pays : United States

Informations de copyright

© 2021, Soveg et al.

Déclaration de conflit d'intérêts

FS, JS, NG, KC, JS, EP, AK, AF, SZ, KE, JR, TH, SO, JC, EM, AS, UM, CS, JP, CB, EA, JH, VM, SS, JW, DS, JB, JB, MG, RS No competing interests declared

Références

J Infect Dis. 2015 Dec 15;212(12):2011-20
pubmed: 26063222
Virology. 2011 Jan 20;409(2):262-70
pubmed: 21056894
J Clin Immunol. 2021 Apr;41(3):536-544
pubmed: 33616813
mBio. 2016 Mar 29;7(2):e00258
pubmed: 27025250
J Virol. 2010 Nov;84(22):11898-904
pubmed: 20844035
Genome Biol. 2016 Nov 29;17(1):246
pubmed: 27899133
Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):2241-6
pubmed: 26858407
J Virol. 2007 Dec;81(23):12720-9
pubmed: 17804500
Neurology. 2010 Aug 3;75(5):411-8
pubmed: 20679634
Science. 2015 Oct 30;350(6260):568-71
pubmed: 26405230
mBio. 2019 Jun 11;10(3):
pubmed: 31186324
Nat Immunol. 2020 Jan;21(1):17-29
pubmed: 31819255
PLoS Biol. 2020 Jun 8;18(6):e3000715
pubmed: 32511245
PLoS Pathog. 2016 Feb 10;12(2):e1005428
pubmed: 26863439
J Gastroenterol Hepatol. 2011 May;26(5):843-50
pubmed: 21182542
Nat Rev Mol Cell Biol. 2016 Feb;17(2):110-22
pubmed: 26790532
N Engl J Med. 2020 May 7;382(19):1787-1799
pubmed: 32187464
BMC Cell Biol. 2014 Sep 09;15:33
pubmed: 25205466
Sci Rep. 2016 Sep 29;6:34379
pubmed: 27682269
Cell Host Microbe. 2007 Oct 11;2(4):229-39
pubmed: 18005741
Viruses. 2014 Jul 22;6(7):2826-57
pubmed: 25054883
Sci Rep. 2017 Dec 14;7(1):17571
pubmed: 29242559
J Immunol. 2009 Dec 15;183(12):8035-43
pubmed: 19923450
Bioinformatics. 2017 Feb 15;33(4):594-595
pubmed: 27742697
Cell Host Microbe. 2019 Feb 13;25(2):336-343.e4
pubmed: 30713099
Nat Rev Immunol. 2014 Aug;14(8):521-8
pubmed: 25033909
Nature. 1987 Dec 10-16;330(6148):587-8
pubmed: 2825034
Cell Host Microbe. 2012 Jun 14;11(6):607-16
pubmed: 22704621
Science. 2014 Mar 14;343(6176):1244-8
pubmed: 24578532
Mol Cell. 2019 May 16;74(4):801-815.e6
pubmed: 30952515
mBio. 2018 Apr 17;9(2):
pubmed: 29666283
Nat Immunol. 2019 Dec;20(12):1610-1620
pubmed: 31740798
J Biol Chem. 1997 Dec 26;272(52):33220-6
pubmed: 9407111
Structure. 2015 May 5;23(5):851-862
pubmed: 25892109
PLoS Pathog. 2009 Feb;5(2):e1000321
pubmed: 19247438
PLoS Genet. 2017 Jun 22;13(6):e1006820
pubmed: 28640813
Nature. 2021 Mar;591(7848):92-98
pubmed: 33307546
Cell. 2019 Jul 11;178(2):275-289.e16
pubmed: 31204099
Proc Natl Acad Sci U S A. 2006 May 2;103(18):7100-5
pubmed: 16627618
J Biol Chem. 1999 Sep 3;274(36):25535-42
pubmed: 10464285
J Mol Evol. 2009 Dec;69(6):612-24
pubmed: 19904482
Am J Hum Genet. 2005 Apr;76(4):623-33
pubmed: 15732009
Cell Rep. 2020 Oct 6;33(1):108234
pubmed: 32979938
Chem Biol Drug Des. 2010 Dec;76(6):460-71
pubmed: 21040496
Nat Rev Microbiol. 2016 Jun;14(6):360-73
pubmed: 27174148
Vaccine. 2011 Nov 8;29(48):8988-97
pubmed: 21939710
Cell Rep. 2017 Feb 28;18(9):2113-2123
pubmed: 28249158
Biochemistry. 2005 Nov 22;44(46):15314-24
pubmed: 16285735
J Exp Med. 2021 Apr 5;218(4):
pubmed: 33544838
J Virol. 2014 Dec;88(24):14222-31
pubmed: 25275129
J Virol. 1997 Sep;71(9):6650-61
pubmed: 9261387
Nature. 2007 Aug 16;448(7155):816-9
pubmed: 17653195

Auteurs

Frank W Soveg (FW)

Department of Immunology, School of Medicine, University of Washington, Seattle, United States.
Center for Innate Immunity and Immune Disease, University of Washington, Seattle, United States.

Johannes Schwerk (J)

Department of Immunology, School of Medicine, University of Washington, Seattle, United States.
Center for Innate Immunity and Immune Disease, University of Washington, Seattle, United States.

Nandan S Gokhale (NS)

Department of Immunology, School of Medicine, University of Washington, Seattle, United States.
Center for Innate Immunity and Immune Disease, University of Washington, Seattle, United States.

Karen Cerosaletti (K)

Benaroya Research Institute at Virginia Mason, Seattle, United States.

Julian R Smith (JR)

Department of Immunology, School of Medicine, University of Washington, Seattle, United States.
Center for Innate Immunity and Immune Disease, University of Washington, Seattle, United States.

Erola Pairo-Castineira (E)

Roslin Institute, University of Edinburgh, Edinburgh, United Kingdom.

Alison M Kell (AM)

Department of Immunology, School of Medicine, University of Washington, Seattle, United States.
Center for Innate Immunity and Immune Disease, University of Washington, Seattle, United States.
Department of Molecular Genetics and Microbiology, School of Medicine, University of New Mexico, Albuquerque, United States.

Adriana Forero (A)

Department of Immunology, School of Medicine, University of Washington, Seattle, United States.
Center for Innate Immunity and Immune Disease, University of Washington, Seattle, United States.
Department of Microbial Infection and Immunity, College of Medicine, The Ohio State University, Columbus, United States.

Shivam A Zaver (SA)

Department of Microbiology, School of Medicine, University of Washington, Seattle, United States.

Katharina Esser-Nobis (K)

Department of Immunology, School of Medicine, University of Washington, Seattle, United States.
Center for Innate Immunity and Immune Disease, University of Washington, Seattle, United States.

Justin A Roby (JA)

Department of Immunology, School of Medicine, University of Washington, Seattle, United States.
Center for Innate Immunity and Immune Disease, University of Washington, Seattle, United States.

Tien-Ying Hsiang (TY)

Department of Immunology, School of Medicine, University of Washington, Seattle, United States.
Center for Innate Immunity and Immune Disease, University of Washington, Seattle, United States.

Snehal Ozarkar (S)

Department of Immunology, School of Medicine, University of Washington, Seattle, United States.
Center for Innate Immunity and Immune Disease, University of Washington, Seattle, United States.

Jonathan M Clingan (JM)

Department of Immunology, School of Medicine, University of Washington, Seattle, United States.
Center for Innate Immunity and Immune Disease, University of Washington, Seattle, United States.

Eileen T McAnarney (ET)

Department of Microbiology and Immunology, University of Texas Medical Center, Galveston, United States.

Amy El Stone (AE)

Department of Immunology, School of Medicine, University of Washington, Seattle, United States.
Center for Innate Immunity and Immune Disease, University of Washington, Seattle, United States.
Department of Basic Sciences, College of Osteopathic Medicine, Touro University Nevada, Henderson, United States.

Uma Malhotra (U)

Department of Infectious Disease, Virginia Mason Medical Center, Seattle, United States.
Department of Medicine, Section of Infectious Diseases, University of Washington, Seattle, United States.

Cate Speake (C)

Benaroya Research Institute at Virginia Mason, Seattle, United States.

Joseph Perez (J)

Cancer Virology Program, University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, United States.

Chiraag Balu (C)

Department of Immunology, School of Medicine, University of Washington, Seattle, United States.
Center for Innate Immunity and Immune Disease, University of Washington, Seattle, United States.

Eric J Allenspach (EJ)

Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, United States.

Jennifer L Hyde (JL)

Department of Microbial Infection and Immunity, College of Medicine, The Ohio State University, Columbus, United States.

Vineet D Menachery (VD)

Department of Microbiology and Immunology, University of Texas Medical Center, Galveston, United States.

Saumendra N Sarkar (SN)

Cancer Virology Program, University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, United States.

Joshua J Woodward (JJ)

Center for Innate Immunity and Immune Disease, University of Washington, Seattle, United States.
Department of Microbiology, School of Medicine, University of Washington, Seattle, United States.

Daniel B Stetson (DB)

Department of Immunology, School of Medicine, University of Washington, Seattle, United States.
Center for Innate Immunity and Immune Disease, University of Washington, Seattle, United States.

John Kenneth Baillie (JK)

Roslin Institute, University of Edinburgh, Edinburgh, United Kingdom.
MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh, United Kingdom.

Jane H Buckner (JH)

Benaroya Research Institute at Virginia Mason, Seattle, United States.

Michael Gale (M)

Department of Immunology, School of Medicine, University of Washington, Seattle, United States.
Center for Innate Immunity and Immune Disease, University of Washington, Seattle, United States.

Ram Savan (R)

Department of Immunology, School of Medicine, University of Washington, Seattle, United States.
Center for Innate Immunity and Immune Disease, University of Washington, Seattle, United States.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female
Humans United States Aged Cross-Sectional Studies Medicare Part C
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH