Two polyurethanases PueA and PueB are major extracellular lipases partly secreted by the mediation of their cognate ABC exporter AprDEF in Pseudomonas protegens Pf-5.
Pseudomonas protegens
ABC exporter
extracellular lipase
polyurethanase
subfamily I.3 lipase
Journal
Letters in applied microbiology
ISSN: 1472-765X
Titre abrégé: Lett Appl Microbiol
Pays: England
ID NLM: 8510094
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
revised:
18
07
2021
received:
26
04
2021
accepted:
22
07
2021
pubmed:
4
8
2021
medline:
3
11
2021
entrez:
3
8
2021
Statut:
ppublish
Résumé
Two polyurethanases PueA and PueB from Pseudomonas protegens Pf-5 have been reported to have hydrolytic activity against synthetic p-nitrophenyl palmitate of lipase substrate, and PueA may play a more effective role in this activity. However, it is still unknown whether PueA and PueB play similar parts in the lipase activity against natural acylglycerols and achieve the extracellular secretion via their cognate ABC exporter AprDEF. In this study, we investigated these questions through the construction of four markerless deletion mutants in Pf5139 (Δupp derivative of Pf-5), two heterologous co-expression strains and their three control strains in lipase-free Escherichia coli BL21(DE3), and detected their lipase activities by the tributyrin plate assay and the liquid culture assay. The results showed that PueA and PueB, classified as subfamily I.3 lipases, are major extracellular lipases involved in the uptake of oil in Pf-5, and PueA plays a leading role in extracellular lipase activity. In addition, the extracellular secretion of PueA and PueB can be partly mediated via AprDEF in Pf-5 and BL21(DE3). Finally, PueA and PueB are also able to achieve the extracellular secretion without the assistance of AprDEF in Pf-5 and BL21(DE3).
Substances chimiques
Bacterial Proteins
0
Lipase
EC 3.1.1.3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
652-657Subventions
Organisme : National Natural Science Foundation of China
ID : 31760534
Organisme : Natural Science Foundation of Jiangxi Province
ID : 20202BAB213022
Informations de copyright
© 2021 The Society for Applied Microbiology.
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