Large Drosophila germline piRNA clusters are evolutionarily labile and dispensable for transposon regulation.
Animals
Animals, Genetically Modified
Argonaute Proteins
/ genetics
Chromosome Deletion
Chromosomes, Insect
DNA Transposable Elements
Drosophila Proteins
/ genetics
Drosophila melanogaster
/ genetics
Evolution, Molecular
Female
Fertility
/ genetics
Gene Expression Regulation, Developmental
Multigene Family
Ovary
/ metabolism
RNA Stability
RNA, Small Interfering
/ genetics
chromosome inversions
flamenco
fruit fly
gene silencing
germ cells
gypsy12
heterochromatin
position-effect variegation
sterility
transposable elements
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
07 10 2021
07 10 2021
Historique:
received:
05
02
2021
revised:
23
05
2021
accepted:
10
07
2021
pubmed:
6
8
2021
medline:
31
12
2021
entrez:
5
8
2021
Statut:
ppublish
Résumé
PIWI proteins and their guiding Piwi-interacting small RNAs (piRNAs) are crucial for fertility and transposon defense in the animal germline. In most species, the majority of piRNAs are produced from distinct large genomic loci, called piRNA clusters. It is assumed that germline-expressed piRNA clusters, particularly in Drosophila, act as principal regulators to control transposons dispersed across the genome. Here, using synteny analysis, we show that large clusters are evolutionarily labile, arise at loci characterized by recurrent chromosomal rearrangements, and are mostly species-specific across the Drosophila genus. By engineering chromosomal deletions in D. melanogaster, we demonstrate that the three largest germline clusters, which account for the accumulation of >40% of all transposon-targeting piRNAs in ovaries, are neither required for fertility nor for transposon regulation in trans. We provide further evidence that dispersed elements, rather than the regulatory action of large Drosophila germline clusters in trans, may be central for transposon defense.
Identifiants
pubmed: 34352205
pii: S1097-2765(21)00580-3
doi: 10.1016/j.molcel.2021.07.011
pmc: PMC8516431
pii:
doi:
Substances chimiques
Argonaute Proteins
0
DNA Transposable Elements
0
Drosophila Proteins
0
RNA, Small Interfering
0
piwi protein, Drosophila
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3965-3978.e5Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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