Cross-Circulation for Extracorporeal Liver Support in a Swine Model.
Journal
ASAIO journal (American Society for Artificial Internal Organs : 1992)
ISSN: 1538-943X
Titre abrégé: ASAIO J
Pays: United States
ID NLM: 9204109
Informations de publication
Date de publication:
01 04 2022
01 04 2022
Historique:
pubmed:
6
8
2021
medline:
2
4
2022
entrez:
5
8
2021
Statut:
ppublish
Résumé
Although machine perfusion has gained momentum as an organ preservation technique in liver transplantation, persistent organ shortages and high waitlist mortality highlight unmet needs for improved organ salvage strategies. Beyond preservation, extracorporeal organ support platforms can also aid the development and evaluation of novel therapeutics. Here, we report the use of veno-arterial-venous (V-AV) cross-circulation (XC) with a swine host to provide normothermic support to extracorporeal livers. Functional, biochemical, and morphological analyses of the extracorporeal livers and swine hosts were performed over 12 hours of support. Extracorporeal livers maintained synthetic function through alkaline bile production and metabolic activity through lactate clearance and oxygen consumption. Beyond initial reperfusion, no biochemical evidence of hepatocellular injury was observed. Histopathologic injury scoring showed improvements in sinusoidal dilatation and composite acute injury scores after 12 hours. Swine hosts remained hemodynamically stable throughout XC support. Altogether, these outcomes demonstrate the feasibility of using a novel V-AV XC technique to provide support for extracorporeal livers in a swine model. V-AV XC has potential applications as a translational research platform and clinical biotechnology for donor organ salvage.
Identifiants
pubmed: 34352819
doi: 10.1097/MAT.0000000000001543
pii: 00002480-202204000-00017
pmc: PMC9984766
mid: NIHMS1811635
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
561-570Subventions
Organisme : NIBIB NIH HHS
ID : P41 EB027062
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL120046
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL140231
Pays : United States
Organisme : NHLBI NIH HHS
ID : U01 HL134760
Pays : United States
Informations de copyright
Copyright © ASAIO 2021.
Déclaration de conflit d'intérêts
Disclosures: The authors have no conflicts of interest to report.
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