Chromatin remodeler INO80 mediates trophectoderm permeability barrier to modulate morula-to-blastocyst transition.

ATPases Associated with Diverse Cellular Activities / genetics Animals Blastocyst / physiology Chromatin / metabolism DNA-Binding Proteins / genetics Embryo Culture Techniques / veterinary Fertilization in Vitro Gene Expression Regulation / physiology Morula / physiology Oocytes / physiology Permeability Swine
Blastocyst INO80 Permeability Tight junction Trophectoderm

Journal

Zoological research
ISSN: 2095-8137
Titre abrégé: Zool Res
Pays: China
ID NLM: 101697192

Informations de publication

Date de publication:
18 Sep 2021
Historique:
entrez: 6 8 2021
pubmed: 7 8 2021
medline: 19 8 2021
Statut: ppublish

Résumé

Inositol requiring mutant 80 (INO80) is a chromatin remodeler that regulates pluripotency maintenance of embryonic stem cells and reprogramming of somatic cells into pluripotent stem cells. However, the roles and mechanisms of INO80 in porcine pre-implantation embryonic development remain largely unknown. Here, we show that INO80 modulates trophectoderm epithelium permeability to promote porcine blastocyst development. The INO80 protein is highly expressed in the nuclei during morula-to-blastocyst transition. Functional studies revealed that RNA interference (RNAi)-mediated knockdown of INO80蛋白是一种染色质重塑因子，参与调控胚胎干细胞多能性的维持以及体细胞诱导为多能干细胞的重编程过程。然而，INO80在猪着床前胚胎发育中的作用及机制尚不清楚。该研究中，我们证实INO80调节滋养层细胞的渗透能力以促进猪囊胚发育。在桑椹胚向囊胚转变过程，INO80蛋白在胚胎细胞核中呈高表达。功能研究证明RNA干扰介导的INO80敲低导致囊胚形成失败，并破坏囊胚中内细胞团和滋养层细胞谱系的分布。单胚胎RNA测序分析揭示INO80调控许多与囊胚细胞谱系分化、紧密连接组装和囊胚腔液体积累等基因的表达。细胞渗透性分析证实INO80敲低囊胚滋养层细胞之间的细胞封闭发生缺陷。对照组和INO80敲低组8-细胞胚胎的聚合试验表明，通过补偿缺陷型滋养层细胞可以诱导INO80敲低胚胎发育成囊胚并恢复正常的细胞谱系分布。因此，以上研究结果证明染色质重塑因子INO80通过调控细胞谱系特化、紧密连接组装和液体聚集等关键基因的表达以促进囊胚发育。.

Autres résumés

Type: Publisher (chi)
INO80蛋白是一种染色质重塑因子，参与调控胚胎干细胞多能性的维持以及体细胞诱导为多能干细胞的重编程过程。然而，INO80在猪着床前胚胎发育中的作用及机制尚不清楚。该研究中，我们证实INO80调节滋养层细胞的渗透能力以促进猪囊胚发育。在桑椹胚向囊胚转变过程，INO80蛋白在胚胎细胞核中呈高表达。功能研究证明RNA干扰介导的INO80敲低导致囊胚形成失败，并破坏囊胚中内细胞团和滋养层细胞谱系的分布。单胚胎RNA测序分析揭示INO80调控许多与囊胚细胞谱系分化、紧密连接组装和囊胚腔液体积累等基因的表达。细胞渗透性分析证实INO80敲低囊胚滋养层细胞之间的细胞封闭发生缺陷。对照组和INO80敲低组8-细胞胚胎的聚合试验表明，通过补偿缺陷型滋养层细胞可以诱导INO80敲低胚胎发育成囊胚并恢复正常的细胞谱系分布。因此，以上研究结果证明染色质重塑因子INO80通过调控细胞谱系特化、紧密连接组装和液体聚集等关键基因的表达以促进囊胚发育。.

Identifiants

DOI: 10.24272/j.issn.2095-8137.2021.075 PMID: 34355875 PMC: PMC8455466
pubmed: 34355875
doi: 10.24272/j.issn.2095-8137.2021.075
pmc: PMC8455466
pii:
doi:

Substances chimiques

Chromatin 0
DNA-Binding Proteins 0
ATPases Associated with Diverse Cellular Activities EC 3.6.4.-

Banques de données

GEO
['GSE176436']

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

562-573

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Auteurs

Zu-Bing Cao (ZB)

Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.

Di Gao (D)

Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.

Hui-Qun Yin (HQ)

Reproductive Medicine Center, 901st Hospital, Hefei, Anhui 230031, China.

Hui Li (H)

Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.

Teng-Teng Xu (TT)

Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.

Meng-Ya Zhang (MY)

Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.

Xin Wang (X)

Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.

Qiu-Chen Liu (QC)

Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.

Ye-Lian Yan (YL)

Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.

Yang-Yang Ma (YY)

Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.

Tong Yu (T)

Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.

Yun-Sheng Li (YS)

Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.

Yun-Hai Zhang (YH)

Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China. E-mail: yunhaizhang@ahau.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de transport › Protéines AAA › ATPases associated with diverse cellular activities › Chromatin remodeler INO80 mediates...
questionsmedicales.fr › Eucaryotes › Animaux › Chromatin remodeler INO80 mediates...
questionsmedicales.fr › Structures de l'embryon › Embryon de mammifère › Blastocyste › Chromatin remodeler INO80 mediates...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Structures de chromosome › Chromatine › Chromatin remodeler INO80 mediates...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de liaison à l'ADN › Chromatin remodeler INO80 mediates...
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture d'embryons › Chromatin remodeler INO80 mediates...
questionsmedicales.fr › Techniques d'investigation › Techniques de reproduction › Techniques de reproduction assistée › Fécondation in vitro › Chromatin remodeler INO80 mediates...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Chromatin remodeler INO80 mediates...
questionsmedicales.fr › Structures de l'embryon › Morula › Chromatin remodeler INO80 mediates...
questionsmedicales.fr › Cellules › Cellules germinales › Ovocytes › Chromatin remodeler INO80 mediates...
questionsmedicales.fr › Phénomènes chimiques › Perméabilité › Chromatin remodeler INO80 mediates...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › Chromatin remodeler INO80 mediates...