BARBEKO'ing in the lab: Versatile CRISPR screens with barcoded base editors.

CRISPR-Cas Systems Clustered Regularly Interspaced Short Palindromic Repeats Cytosine Gene Editing Reproducibility of Results

Journal

Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571

Informations de publication

Date de publication:
05 08 2021
Historique:
entrez: 6 8 2021
pubmed: 7 8 2021
medline: 12 8 2021
Statut: ppublish

Résumé

Xu et al. (2021) describe a novel two-pronged CRISPR screen, termed BARBEKO, by coupling cytosine base editors and internally barcoded sgRNAs to eliminate double-stranded break-induced toxicity, enable high multiplicities of infection, and ensure experimental reproducibility.

Identifiants

DOI: 10.1016/j.molcel.2021.07.007 PMID: 34358458
pubmed: 34358458
pii: S1097-2765(21)00575-X
doi: 10.1016/j.molcel.2021.07.007
pii:
doi:

Substances chimiques

Cytosine 8J337D1HZY

Types de publication

Journal Article Research Support, Non-U.S. Gov't Comment

Langues

eng

Sous-ensembles de citation

IM

Pagination

3046-3047

Commentaires et corrections

Type : CommentOn

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Auteurs

Pranam Chatterjee (P)

Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, USA; Department of Genetics, Harvard Medical School, Cambridge, MA, USA; Center for Bits and Atoms, Cambridge, MA, USA; MIT Media Lab, Massachusetts Institute of Technology, Cambridge, MA, USA. Electronic address: pranam@mit.edu.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › BARBEKO'ing in the lab: Versatile CRISPR...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Séquences répétées en tandem › Clustered regularly interspaced short palindromic repeats › BARBEKO'ing in the lab: Versatile CRISPR...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Pyrimidines › Pyrimidinones › Cytosine › BARBEKO'ing in the lab: Versatile CRISPR...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › BARBEKO'ing in the lab: Versatile CRISPR...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › BARBEKO'ing in the lab: Versatile CRISPR...