Adipose-Derived Stem Cell Features and MCF-7.
Adipose Tissue
/ cytology
Autophagosomes
/ metabolism
Autophagy
/ genetics
Bromodeoxyuridine
/ metabolism
Cell Proliferation
Cell Shape
Cell Survival
Culture Media
Cyclin-Dependent Kinase Inhibitor p21
/ metabolism
Epigenesis, Genetic
Humans
MCF-7 Cells
Middle Aged
Polyamines
/ metabolism
Stem Cells
/ cytology
Tumor Suppressor Protein p53
/ metabolism
bcl-2-Associated X Protein
/ metabolism
adipose-derived stem cells
autophagy
cell proliferation
cellular mechanisms
epigenetics
stemness genes
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
11 07 2021
11 07 2021
Historique:
received:
08
06
2021
revised:
09
07
2021
accepted:
09
07
2021
entrez:
7
8
2021
pubmed:
8
8
2021
medline:
27
10
2021
Statut:
epublish
Résumé
Human adipose tissue-derived stem cells (hADSCs) are highly suitable for regeneration therapies being easily collected and propagated in vitro. The effects of different external factors and culturing conditions are able to affect hADSC proliferation, senescence, differentiation, and migration, even at the molecular level. In the present paper, we exposed hADSCs to an exhausted medium from the breast cancer cell line (MCF-7) to evaluate whether the soluble factors released by these cells may be able to induce changes in stem cell behavior. In particular, we investigated the expression of stemness-related genes (OCT4; Sox 2; Nanog), the cell-cycle regulators p21 (WAF1/CIP1) p53, epigenetic markers (DNMT1 and Sirt1), and autophagy-related proteins. From our results, we can infer that the exhausted medium from MCF-7 is able to influence the hADSCs behavior increasing the expression of stemness-related genes, cell proliferation, and autophagy. Polyamines detectable in MCF-7 exhausted medium could be related to the higher proliferation capability observed in hADSCs, suggesting direct crosstalk between these molecules and the observed changes in stem cell potency.
Identifiants
pubmed: 34359925
pii: cells10071754
doi: 10.3390/cells10071754
pmc: PMC8307920
pii:
doi:
Substances chimiques
Culture Media
0
Cyclin-Dependent Kinase Inhibitor p21
0
Polyamines
0
Tumor Suppressor Protein p53
0
bcl-2-Associated X Protein
0
Bromodeoxyuridine
G34N38R2N1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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