Microfabrication of a Chamber for High-Resolution, In Situ Imaging of the Whole Root for Plant-Microbe Interactions.
GFP-like proteins
fluorescently-tagged bacteria
imaging
microfabrication
microscopy
plant–microbe interactions
rhizosphere
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
23 Jul 2021
23 Jul 2021
Historique:
received:
30
06
2021
revised:
17
07
2021
accepted:
18
07
2021
entrez:
7
8
2021
pubmed:
8
8
2021
medline:
17
8
2021
Statut:
epublish
Résumé
Fabricated ecosystems (EcoFABs) offer an innovative approach to in situ examination of microbial establishment patterns around plant roots using nondestructive, high-resolution microscopy. Previously high-resolution imaging was challenging because the roots were not constrained to a fixed distance from the objective. Here, we describe a new 'Imaging EcoFAB' and the use of this device to image the entire root system of growing
Identifiants
pubmed: 34360661
pii: ijms22157880
doi: 10.3390/ijms22157880
pmc: PMC8348081
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : U.S. Department of Energy
ID : DE-AC02-05CH11231
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