Generation of an induced pluripotent stem cell line (ZJUi007-A) from a 11-year-old patient of Fabry disease.

Cell Differentiation Cellular Reprogramming Child Fabry Disease / genetics Humans Induced Pluripotent Stem Cells Kruppel-Like Factor 4 Leukocytes, Mononuclear Mutation Plasmids

Journal

Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957

Informations de publication

Date de publication:
08 2021
Historique:
received: 16 06 2021
revised: 09 07 2021
accepted: 15 07 2021
pubmed: 9 8 2021
medline: 27 10 2021
entrez: 8 8 2021
Statut: ppublish

Résumé

PBMCs were collected from a patient with a novel GLA gene mutation (c.140G > A) which contributed to Fabry disease. Subsequently, an induced pluripotent stem cell (iPSC) line was derived using an episomal reprogramming method that transfer the reprogramming plasmids expressing OCT3/4, SOX2, KLF4, LIN28 and L-MYC into the PBMCs. The expected mutation in the iPSC line was confirmed by Sanger sequencing, while the pluripotency status was validated by immunofluorescence assay and flow cytometry for pluripotency markers, as well as teratoma formation.

Identifiants

DOI: 10.1016/j.scr.2021.102475 PMID: 34365103
pubmed: 34365103
pii: S1873-5061(21)00322-6
doi: 10.1016/j.scr.2021.102475
pii:
doi:

Types de publication

Case Reports Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

102475

Informations de copyright

Copyright © 2021. Published by Elsevier B.V.

Auteurs

Yuqing Zhu (Y)

Center for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, & The First Affiliated Hospital, College of Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.

Li Zhang (L)

Center for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, & The First Affiliated Hospital, College of Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.

Gang Wang (G)

National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210093, China.

Jing Zhao (J)

Center for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, & The First Affiliated Hospital, College of Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.

Xiaolin Hou (X)

Center for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, & The First Affiliated Hospital, College of Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.

Hangdi Wu (H)

Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.

Yuming Xu (Y)

Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.

Jianhua Mao (J)

Department of Nephrology, The Children Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, China.

Zhihong Liu (Z)

Zhejiang Laboratory for Systems and Precision Medicine, Zhejiang University Medical Center, Hangzhou 311121, China; National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210093, China.

Jin Zhang (J)

Center for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, & The First Affiliated Hospital, College of Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China; Zhejiang Laboratory for Systems and Precision Medicine, Zhejiang University Medical Center, Hangzhou 311121, China; Institute of Hematology, Zhejiang University, Hangzhou, Zhejiang 310058, China.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Generation of an induced pluripotent stem cell...
questionsmedicales.fr › Phénomènes génétiques › Reprogrammation cellulaire › Generation of an induced pluripotent stem cell...
questionsmedicales.fr › Personnes › Tranches d'âge › Enfant › Generation of an induced pluripotent stem cell...
questionsmedicales.fr › Maladies métaboliques et nutritionnelles › Maladies métaboliques › Erreurs innées du métabolisme › Maladies lysosomiales › Maladies neurologiques de surcharge lysosomiale › Sphingolipidoses › Maladie de Fabry › Generation of an induced pluripotent stem cell...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Generation of an induced pluripotent stem cell...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches pluripotentes induites › Generation of an induced pluripotent stem cell...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription Krüppel-like › Facteur-4 de type Kruppel › Generation of an induced pluripotent stem cell...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Generation of an induced pluripotent stem cell...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Generation of an induced pluripotent stem cell...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Plasmides › Generation of an induced pluripotent stem cell...