The diarrhetic shellfish-poisoning toxin, okadaic acid, provokes gastropathy, dysbiosis and susceptibility to bacterial infection in a non-rodent bioassay, Galleria mellonella.
16S (V3–V4) rRNA microbiome
Food poisoning
Histopathology
Immune-compromised
Marine toxins
In vivo model
Journal
Archives of toxicology
ISSN: 1432-0738
Titre abrégé: Arch Toxicol
Pays: Germany
ID NLM: 0417615
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
06
07
2021
accepted:
04
08
2021
pubmed:
11
8
2021
medline:
29
1
2022
entrez:
10
8
2021
Statut:
ppublish
Résumé
Diarrhetic shellfish-poisoning (DSP) toxins such as okadaic acid and dinophysistoxins harm the human gastrointestinal tract, and therefore, their levels are regulated to an upper limit of 160 μg per kg tissue to protect consumers. Rodents are used routinely for risk assessment and studies concerning mechanisms of toxicity, but there is a general move toward reducing and replacing vertebrates for these bioassays. We have adopted insect larvae of the wax moth Galleria mellonella as a surrogate toxicology model. We treated larvae with environmentally relevant doses of okadaic acid (80-400 μg/kg) via intrahaemocoelic injection or gavage to determine marine toxin-related health decline: (1) whether pre-exposure to a sub-lethal dose of toxin (80 μg/kg) enhances susceptibility to bacterial infection, or (2) alters tissue pathology and bacterial community (microbiome) composition of the midgut. A sub-lethal dose of okadaic acid (80 μg/kg) followed 24 h later by bacterial inoculation (2 × 10
Identifiants
pubmed: 34374792
doi: 10.1007/s00204-021-03132-x
pii: 10.1007/s00204-021-03132-x
pmc: PMC8448676
doi:
Substances chimiques
Okadaic Acid
1W21G5Q4N2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3361-3376Subventions
Organisme : interreg
ID : Bluefish
Organisme : european social fund
ID : KESS2
Informations de copyright
© 2021. The Author(s).
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