A modified Tet-ON system minimizing leaky expression for cell-type specific gene induction in medaka fish.
doxycycline
genetically modified
retinal rod photoreceptor cells
trans-activators
transgenes
Journal
Development, growth & differentiation
ISSN: 1440-169X
Titre abrégé: Dev Growth Differ
Pays: Japan
ID NLM: 0356504
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
revised:
28
06
2021
received:
14
05
2021
accepted:
27
07
2021
pubmed:
11
8
2021
medline:
18
1
2022
entrez:
10
8
2021
Statut:
ppublish
Résumé
The Tet-ON system is an important molecular tool for temporally and spatially-controlled inducible gene expression. Here, we developed a Tet-ON system to induce transgene expression specifically in the rod photoreceptors of medaka fish. Our modified reverse tetracycline-controlled transcriptional transactivator (rtTAm) with 5 amino acid substitutions dramatically improved the leakiness of the transgene in medaka fish. We generated a transgenic line carrying a self-reporting vector with the rtTAm gene driven by the Xenopus rhodopsin promoter and a tetracycline response element (TRE) followed by the green fluorescent protein (GFP) gene. We demonstrated that GFP fluorescence was restricted to the rod photoreceptors in the presence of doxycycline in larval fish (9 days post-fertilization). The GFP fluorescence intensity was enhanced with longer durations of doxycycline treatment up to 72 h and in a dose-dependent manner (5-45 μg/ml). These findings demonstrate that the Tet-ON system using rtTAm allows for spatiotemporal control of transgene expression, at least in the rod photoreceptors, in medaka fish.
Substances chimiques
Trans-Activators
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
397-405Subventions
Organisme : Japan Society for the Promotion of Science
ID : 18H02479
Organisme : Japan Society for the Promotion of Science
ID : 18K06349
Organisme : Japan Society for the Promotion of Science
ID : 20H04925
Organisme : Japan Society for the Promotion of Science
ID : 21H04773
Organisme : Japan Society for the Promotion of Science
ID : KAKENHI Grant number 21K09721
Organisme : Mitsubishi Foundation
Organisme : Takeda Science Foundation
Informations de copyright
© 2021 Japanese Society of Developmental Biologists.
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