Role of Mitochondrial Dynamics in Microglial Activation and Metabolic Switch.
Animals
Animals, Newborn
Calcium
/ metabolism
Cells, Cultured
Dynamins
/ metabolism
Interferon-gamma
/ pharmacology
Lipopolysaccharides
/ pharmacology
Membrane Potential, Mitochondrial
/ drug effects
Microglia
/ cytology
Mitochondria
/ drug effects
Mitochondrial Dynamics
/ drug effects
Oxidative Phosphorylation
/ drug effects
Proton-Translocating ATPases
/ metabolism
Quinazolinones
/ pharmacology
Rats
Reactive Oxygen Species
/ metabolism
Journal
ImmunoHorizons
ISSN: 2573-7732
Titre abrégé: Immunohorizons
Pays: United States
ID NLM: 101708159
Informations de publication
Date de publication:
10 08 2021
10 08 2021
Historique:
received:
19
07
2021
accepted:
20
07
2021
entrez:
11
8
2021
pubmed:
12
8
2021
medline:
29
1
2022
Statut:
epublish
Résumé
Microglia act as sensors of injury in the brain, favoring its homeostasis. Their activation and polarization toward a proinflammatory phenotype are associated with injury and disease. These processes are linked to a metabolic reprogramming of the cells, characterized by high rates of glycolysis and suppressed oxidative phosphorylation. This metabolic switch can be reproduced in vitro by microglial stimulation with LPS plus IFN-γ. To understand the mechanisms regulating mitochondrial respiration abolishment, we examined potential alterations in mitochondrial features during this switch using rat primary microglia. Cells did not show any change in mitochondrial membrane potential, suggesting a limited impact in the mitochondrial viability. We provide evidence that reverse operation of F
Identifiants
pubmed: 34376483
pii: immunohorizons.2100068
doi: 10.4049/immunohorizons.2100068
doi:
Substances chimiques
3-(2,4-dichloro-5-methoxyphenyl)-2-sulfanyl-4(3H)-quinazolinone
0
Lipopolysaccharides
0
Quinazolinones
0
Reactive Oxygen Species
0
Interferon-gamma
82115-62-6
Proton-Translocating ATPases
EC 3.6.3.14
Dnm1l protein, rat
EC 3.6.5.5
Dynamins
EC 3.6.5.5
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
615-626Informations de copyright
Copyright © 2021 The Authors.