Combining IL-6 and SARS-CoV-2 RNAaemia-based risk stratification for fatal outcomes of COVID-19.


Journal

PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081

Informations de publication

Date de publication:
2021
Historique:
received: 04 01 2021
accepted: 29 07 2021
entrez: 11 8 2021
pubmed: 12 8 2021
medline: 24 8 2021
Statut: epublish

Résumé

The coronavirus disease 2019 (COVID-19) pandemic rapidly increases the use of mechanical ventilation (MV). Such cases further require extracorporeal membrane oxygenation (ECMO) and have a high mortality. We aimed to identify prognostic biomarkers pathophysiologically reflecting future deterioration of COVID-19. Clinical, laboratory, and outcome data were collected from 102 patients with moderate to severe COVID-19. Interleukin (IL)-6 level and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA copy number in plasma were assessed with ELISA kit and quantitative PCR. Twelve patients died or required ECMO owing to acute respiratory distress syndrome despite the use of MV. Among various variables, a ratio of oxygen saturation to fraction of inspired oxygen (SpO2/FiO2), IL-6, and SARS-CoV-2 RNA on admission before intubation were strongly predictive of fatal outcomes after the MV use. Moreover, among these variables, combining SpO2/FiO2, IL-6, and SARS-CoV-2 RNA showed the highest accuracy (area under the curve: 0.934). In patients with low SpO2/FiO2 (< 261), fatal event-rate after the MV use at the 30-day was significantly higher in patients with high IL-6 (> 49 pg/mL) and SARS-CoV-2 RNAaemia (> 1.5 copies/μL) compared to those with high IL-6 or RNAaemia or without high IL-6 and RNAaemia (88% vs. 22% or 8%, log-rank test P = 0.0097 or P < 0.0001, respectively). Combining SpO2/FiO2 with high IL-6 and SARS-CoV-2 RNAaemia which reflect hyperinflammation and viral overload allows accurately and before intubation identifying COVID-19 patients at high risk for ECMO use or in-hospital death despite the use of MV.

Sections du résumé

BACKGROUND
The coronavirus disease 2019 (COVID-19) pandemic rapidly increases the use of mechanical ventilation (MV). Such cases further require extracorporeal membrane oxygenation (ECMO) and have a high mortality.
OBJECTIVE
We aimed to identify prognostic biomarkers pathophysiologically reflecting future deterioration of COVID-19.
METHODS
Clinical, laboratory, and outcome data were collected from 102 patients with moderate to severe COVID-19. Interleukin (IL)-6 level and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA copy number in plasma were assessed with ELISA kit and quantitative PCR.
RESULTS
Twelve patients died or required ECMO owing to acute respiratory distress syndrome despite the use of MV. Among various variables, a ratio of oxygen saturation to fraction of inspired oxygen (SpO2/FiO2), IL-6, and SARS-CoV-2 RNA on admission before intubation were strongly predictive of fatal outcomes after the MV use. Moreover, among these variables, combining SpO2/FiO2, IL-6, and SARS-CoV-2 RNA showed the highest accuracy (area under the curve: 0.934). In patients with low SpO2/FiO2 (< 261), fatal event-rate after the MV use at the 30-day was significantly higher in patients with high IL-6 (> 49 pg/mL) and SARS-CoV-2 RNAaemia (> 1.5 copies/μL) compared to those with high IL-6 or RNAaemia or without high IL-6 and RNAaemia (88% vs. 22% or 8%, log-rank test P = 0.0097 or P < 0.0001, respectively).
CONCLUSIONS
Combining SpO2/FiO2 with high IL-6 and SARS-CoV-2 RNAaemia which reflect hyperinflammation and viral overload allows accurately and before intubation identifying COVID-19 patients at high risk for ECMO use or in-hospital death despite the use of MV.

Identifiants

pubmed: 34379684
doi: 10.1371/journal.pone.0256022
pii: PONE-D-21-00168
pmc: PMC8357172
doi:

Substances chimiques

Interleukin-6 0
RNA, Viral 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0256022

Déclaration de conflit d'intérêts

The authors have declared that no competing interests exist.

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Auteurs

Ryo Saji (R)

Department of Emergency Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.

Mototsugu Nishii (M)

Department of Emergency Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.

Kazuya Sakai (K)

Department of Emergency Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.

Kei Miyakawa (K)

Department of Microbiology, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.

Yutaro Yamaoka (Y)

Department of Microbiology, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.
Life Science Laboratory, Technology and Development Division, Kanto Chemical Co., Inc., Isehara, Kanagawa, Japan.

Tatsuma Ban (T)

Department of Immunology, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.

Takeru Abe (T)

Department of Advanced Critical Care and Emergency Center, Yokohama City University Medical Center, Yokohama, Kanagawa, Japan.

Yutaro Ohyama (Y)

Department of Emergency Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.

Kento Nakajima (K)

Department of Emergency Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.

Taro Hiromi (T)

Department of Emergency Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.

Reo Matsumura (R)

Department of Emergency Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.

Naoya Suzuki (N)

Department of Critical Care and Emergency Medicine, National Hospital Organization Yokohama Medical Center, Yokohama, Kanagawa, Japan.

Hayato Taniguchi (H)

Department of Advanced Critical Care and Emergency Center, Yokohama City University Medical Center, Yokohama, Kanagawa, Japan.

Tsuyoshi Otsuka (T)

Department of Critical Care and Emergency Medicine, National Hospital Organization Yokohama Medical Center, Yokohama, Kanagawa, Japan.

Yasufumi Oi (Y)

Department of Emergency Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.

Fumihiro Ogawa (F)

Department of Emergency Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.

Munehito Uchiyama (M)

Department of Emergency Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.

Kohei Takahashi (K)

Department of Advanced Critical Care and Emergency Center, Yokohama City University Medical Center, Yokohama, Kanagawa, Japan.

Masayuki Iwashita (M)

Department of Advanced Critical Care and Emergency Center, Yokohama City University Medical Center, Yokohama, Kanagawa, Japan.

Yayoi Kimura (Y)

Department of Advanced Medical Research Center, Yokohama City University, School of Medicine, Yokohama, Kanagawa, Japan.

Satoshi Fujii (S)

Department of Molecular Pathology, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.

Ryosuke Furuya (R)

Department of Critical Care and Emergency Medicine, National Hospital Organization Yokohama Medical Center, Yokohama, Kanagawa, Japan.

Tomohiko Tamura (T)

Department of Immunology, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.
Department of Advanced Medical Research Center, Yokohama City University, School of Medicine, Yokohama, Kanagawa, Japan.

Akihide Ryo (A)

Department of Microbiology, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.
Department of Advanced Medical Research Center, Yokohama City University, School of Medicine, Yokohama, Kanagawa, Japan.

Ichiro Takeuchi (I)

Department of Emergency Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.
Department of Advanced Critical Care and Emergency Center, Yokohama City University Medical Center, Yokohama, Kanagawa, Japan.

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