FACT is recruited to the +1 nucleosome of transcribed genes and spreads in a Chd1-dependent manner.
Chromatin
/ metabolism
Chromatin Assembly and Disassembly
Chromosomal Proteins, Non-Histone
/ metabolism
DNA-Binding Proteins
/ genetics
High Mobility Group Proteins
/ genetics
Histone Chaperones
/ genetics
Histones
/ genetics
Molecular Chaperones
/ metabolism
Nucleosomes
/ metabolism
Protein Binding
RNA Polymerase II
/ metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
/ genetics
Transcription, Genetic
/ genetics
Transcriptional Elongation Factors
/ genetics
Chd1
FACT
Pob3
RNA polymerase II
Spt16
chromatin remodeling
histone chaperone
mathematical modeling
nucleosome unwrapping
single-molecule tracking
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
02 09 2021
02 09 2021
Historique:
received:
02
08
2020
revised:
25
05
2021
accepted:
12
07
2021
pubmed:
12
8
2021
medline:
10
4
2022
entrez:
11
8
2021
Statut:
ppublish
Résumé
The histone chaperone FACT occupies transcribed regions where it plays prominent roles in maintaining chromatin integrity and preserving epigenetic information. How it is targeted to transcribed regions, however, remains unclear. Proposed models include docking on the RNA polymerase II (RNAPII) C-terminal domain (CTD), recruitment by elongation factors, recognition of modified histone tails, and binding partially disassembled nucleosomes. Here, we systematically test these and other scenarios in Saccharomyces cerevisiae and find that FACT binds transcribed chromatin, not RNAPII. Through a combination of high-resolution genome-wide mapping, single-molecule tracking, and mathematical modeling, we propose that FACT recognizes the +1 nucleosome, as it is partially unwrapped by the engaging RNAPII, and spreads to downstream nucleosomes aided by the chromatin remodeler Chd1. Our work clarifies how FACT interacts with genes, suggests a processive mechanism for FACT function, and provides a framework to further dissect the molecular mechanisms of transcription-coupled histone chaperoning.
Identifiants
pubmed: 34380014
pii: S1097-2765(21)00579-7
doi: 10.1016/j.molcel.2021.07.010
pmc: PMC9149603
mid: NIHMS1726948
pii:
doi:
Substances chimiques
CHD1 protein, S cerevisiae
0
Chromatin
0
Chromosomal Proteins, Non-Histone
0
DNA-Binding Proteins
0
FACT protein, S cerevisiae
0
High Mobility Group Proteins
0
Histone Chaperones
0
Histones
0
Molecular Chaperones
0
Nucleosomes
0
Saccharomyces cerevisiae Proteins
0
Transcriptional Elongation Factors
0
RNA Polymerase II
EC 2.7.7.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3542-3559.e11Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM132290
Pays : United States
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S009035/1
Pays : United Kingdom
Organisme : CIHR
ID : MOP-162334
Pays : Canada
Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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